CORE-- MICROSCOPE FACILITY

描述：共焦和电子显微镜设备配备用于 电子显微镜(EM)和光学显微镜(LM) 活细胞成像，包括延时视频和钙成像系统。它 在上一次筹资期间也得到了扩展，包括一台计算机 用于图像处理和分析的成像设备。所有5个项目都将使用 基于LM的系统和3个将使用EM。除了设备，设施还包括 提供培训和技术支持。该设施由莱斯利博士监督 托尔伯特和雇佣了三名工作人员：1)一个硕士级别的高级研究 专家，Jansma女士(60%的PPG努力，40%的司努力) 作为EM和共聚焦显微镜的技术专家，她维护着所有 确定范围，培训新用户，并帮助开发协议。2)兼职 研究技术员，波茨女士(在这里付出20%的努力，在项目2上付出80%的努力) 提供关于共焦和活细胞系统的培训和援助。3)a 兼职计算机专家Yuhas先生(75%致力于PPG，25%致力于 部门)维护计算机、安装升级并对用户进行软件培训。

DESCRIPTION: The Confocal and Electron Microscopy Facility is equipped for electron microscopy (EM) and light microscopy (LM) including confocal and live-cell imaging, including time-lapse video and calcium imaging systems. It has also been expanded during the previous funding period to include a Computer Imaging Facility for image processing and analysis. All 5 projects will use the LM-based systems and 3 will use EM. In addition to the equipment, the facility provides training and technical support. The facility is overseen by Dr. Leslie Tolbert and employs three staff members: 1) a Master's level Senior Research Specialist, Ms. Jansma, (60% effort on PPG and 40% for the Division) technically expert in EM and confocal microscopy, she maintains all of the scopes, trains new users and helps to develop protocols. 2) a part-time research technician, Ms. Potts (20% effort here and 80% effort on Project 2) provides training and assistance with the confocal and live-cell systems. 3) a part-time computer expert, Mr. Yuhas (75% effort on PPG and 25% effort in the Division) maintains computers, installs upgrades and trains users in software.