CORE--MUTANT MOUSE

描述：(改编自申请者S摘要) 变异鼠核心的目标是集中维护和 基因打靶诱变小鼠的选育 (基因敲除小鼠)、基因添加(转基因小鼠)和基因组合。 目前，CORE拥有可用于以下基因的基因敲除小鼠： 神经元型一氧化氮合酶(nNOS或NOS1)，诱导型一氧化氮合酶 (iNOS或NOS2)、内皮型一氧化氮合酶(eNOS或NOS3)、B2 缓激肽受体(B2)和血管紧张素II 2型受体(AT2)。这个 CORE也有三种NOS亚型中的两种被敲除的小鼠 每个成对组合(e/iNOS、e/nNOS和i/nNOS)。一群转基因生物 脑利钠肽启动子驱动的荧光素酶在小鼠体内的表达 (BNP-LUC)可用。表达人类基因的转基因小鼠的创始人 心脏中的血管紧张素II(Ang II)已经产生并增加了 在心脏中表达Ang II的转基因小鼠品系将被进口。 表达牛eNOS的转基因创建者的粗大上肢 已经产生了Henle环。此外，核心将输入基因 组织激肽释放酶(TK)和PGE2受体2型和3型基因敲除小鼠 (分别为EP2和EP3)。核心还将创建各种组合 基因敲除和转基因小鼠，并提供适当的控制 都不能在商业上买到。选择适当的控件是 为每个突变体确定，以便它们的遗传背景将尽可能接近 对实验小鼠越好越好。核心负责提供 向参与调查的人员提供专家建议并建立育种计划 以产生所需数量的实验小鼠和对照组。在……里面 此外，核心将通过以下方式确定所有断奶仔猪的相关基因类型 聚合酶链式反应。近亲交配遗传背景不一致的小鼠通常会 回交到菌株C57BL/6J(B6)，直到达到同源状态(a 至少10个回交世代)。同源基因的小鼠将被培育成 为调查人员提供实验动物。(摘要结束)

DESCRIPTION: (adapted from applicant?s abstract) The objective of the Mutant Mouse Core is to centralize maintenance and breeding of mice with various induced mutations generated by gene targeting (gene knockout mice), gene addition (transgenic mice), and gene combinations. Currently, the core has available gene knockout mice for the following genes: neuronal nitric oxide synthase (nNOS or NOS1), inducible nitric oxide synthase (iNOS or NOS2), endothelial nitric oxide synthase (eNOS or NOS3), B2 bradykinin receptor (B2), and the angiotensin II type 2 receptor (AT2). The core also has available mice with two of the three NOS isoforms knocked out in each pairwise combination (e/iNOS, e/nNOS, and i/nNOS). A colony of transgenic mice with brain natriuretic peptide promoter-driven luciferase expression (BNP-Luc) is available. Founders for transgenic mice expressing human angiotensin II (Ang II) in the heart have been generated and additional transgenic mouse strains expressing Ang II in the heart will be imported. Transgenic founders expressing bovine eNOS in the thick ascending limb of the loop of Henle have been generated. In addition, the Core will import gene knockout mice for tissue kallikrein (TK), and PGE2 receptor type 2 and type 3 (EP2 and EP3 respectively). The Core will also create various combinations of gene knockout and transgenic mice, and provide appropriate controls if they are not commercially available. The choice of appropriate controls is determined for each mutant so that their genetic background will be as close to the experimental mice as possible. The Core is responsible for providing expert advice to participating investigators and setting up breeding schemes to generate the required numbers of experimental mice and controls. In addition, the core will determine the relevant genotypes of all weanlings by PCR. Mice which are not of a uniform inbred genetic background will often be backcrossed to strain C57BL/6J (B6) until congenic status is reached (a minimum of 10 backcross generations). The congenic mice will be bred to provide experimental animals for the investigators. (End of abstract)