CORE--TRANSGENIC MOUSE

描述(申请人提供)：基因小鼠模型的使命 共享资源是为存储区域网络的成员提供集中的资源 安东尼奥癌症研究所的设计、生成和评估 转基因小鼠。共享资源由保罗·哈蒂斯执导， D.V.M.，在生产和分析方面具有丰富的经验 基因敲除小鼠和基因敲除小鼠在癌症研究中的应用。他有 产生并分析了许多基因突变的小鼠，包括DNA修复 基因RAD51、Ku80和BRCA2。此外，哈提斯博士还担任了 Licion Genetics，Inc.，负责鼠标基因敲除单位(Licion Genetics 每年产生数百只基因敲除小鼠)。遗传性小鼠模型 共享资源将是满足不断发展的科学需求的关键 SACI成员在基础研究和翻译研究中的地位。服务包括： 1.基因组改变的设计：协助方案设计 从基本的击倒到范围广泛的更复杂的方法，如 组织特异性和条件性基因敲除。此外，这一核心将 使用小鼠胚胎干细胞提供转基因服务。2. 分离具有所需基因组改变的ES细胞：DNA载体是 将其导入ES细胞，并应用合适的选择介质。 耐药菌落将被分离、扩增和复制。3. 转基因小鼠(杂合子)的产生：ES细胞与 适当的基因改造将被注射到供体囊胚中，并 转移到假孕雌性体内。这些嵌合体小鼠将被培育成 获得转基因杂合子小鼠。在对嵌合体进行基因分型后 后代，转基因小鼠将被交付给研究人员。 4.转基因小鼠分析咨询：一旦 转基因小鼠已经建立，遗传小鼠模型 共享资源将就畜牧业、基因分型和 表型分析。

DESCRIPTION (provided by applicant): The mission of the Genetic Mouse Models Shared Resource is to provide a centralized resource to the members of the San Antonio Cancer Institute for the design, generation and evaluation of genetically altered mice. The shared resource is Directed by Paul Hasty, D.V.M., who has extensive experience in the production and analysis of knockout mice and in the use of knockout mice in cancer research. He has generated and analyzed mice mutated for many genes including the DNA repair genes Rad51, Ku80 and Brca2. In addition, Dr. Hasty was the Director at Lexicon Genetics, Inc., in charge of the mouse knockout unit (Lexicon Genetics generates several hundred knockout mice per year). The Genetic Mouse Models Shared Resource will be essential to meet the developing scientific needs of of SACI memebrs in both basic and translational research. Services include: 1. Design of genomic alteration: Assistance is provided in protocol design from basic knockouts to a wide-range of more sophisticated approaches such as tissue-specific and conditional knockouts. In addition, this Core will provide transgenic services using mouse embryonic stem (ES) cells. 2. Isolation of ES cells with the desired genomic alteration: DNA vectors are transfected into ES cells and the appropriate selection media is applied. Drug-resistant colonies will be isolated, expanded and replica-plated. 3. Generation of genetically altered mice (heterozygotes): ES cells with the appropriate genetic modification will be injected into donor blastocysts and transferred into pseudopregnant females. These chimeric mice will be bred to obtain genetically modified heterozygote mice. After genotyping the chimera's progeny, the genetically modified mice will be delivered to the investigator. 4. Consultation on analysis of genetically altered mice: Once the genetically altered mice have been established, the Genetic Mouse Models Shared Resource will consult with investigators on husbandry, genotyping and phenotypic analysis.