Endothelial Specific Caveolin-1 Overexpression

描述（由申请人提供）：本申请提出利用内皮特异性caveolin-1转基因小鼠研究caveolin-1在血管功能中的作用。目的1围绕转基因小鼠的特性展开，包括确定转基因小鼠的组织分布，以及小窝蛋白-1过表达对组织形态、血压、离体血管对血管收缩剂和血管舒张剂的反应的影响。在目标2中，我建议研究从转基因小鼠中分离的内皮细胞。各种内皮细胞功能的体外实验将进行，包括细胞增殖、迁移和小管样结构形成的实验。目的3研究内皮特异性caveolin-1过表达对体内血管通透性和血管生成的影响。综上所述，本文提出的实验将建立一种新的模型（内皮特异性caveolin-1转基因小鼠）来研究内皮caveolin-1在血流动力学、血管控制、血管通透性和血管生成中的作用。

DESCRIPTION (provided by applicant):The proposal put forth in this application proposes the use of endothelial specific caveolin- 1 transgenic mice to study the role of caveolin-1 in vascular function. Aim 1 centers around the characterization of the transgenic mice, including determination of the tissue distribution of the transgene, and the effect of caveolin-1 overexpression on tissue morphology, blood pressure, the response of isolated blood vessels to vasoconstrictors and vasodilators. In aim 2, I propose to study endothelial cells isolated from the transgenic mice. Various in vitro assays of endothelial cell function will be performed including assays of cell proliferation, migration, and formation of tubule like structures. Aim 3 proposes to study the effect of endothelial specific caveolin-1 overexpression on vascular permeability and angiogenesis in vivo. In summary, the experiments proposed herein will develop a novel model (endothelial specific caveolin-1 transgenic mice) to study the role of endothelial caveolin-1 in hemodynamics, vascular control, vascular permeability, and angiogenesis.