SEQUENCING THE MOUSE GENOME

我们计划实现应用程序中概述的以下目标。在第一年：为覆盖整个小鼠基因组的BAC克隆生成指纹数据，并组装重叠群。使用15,000个探针将指纹重叠群定位到老鼠的身体和遗传学地图上。生成200,000个BAC末端序列。建立一个整合所有遗传、物理和重叠群数据的网站，以帮助协调整个小鼠测序工作。在第二年和第三年：为一半的小鼠基因组生成工作草稿序列。在第一年到第三年：生成115 Mb的完成序列。将这个已完成的序列定位于老鼠研究社区感兴趣的区域。指纹数据将被用来组装重叠群，从中可以选择重叠程度最低的克隆。在其他美国实验室和桑格中心的贡献下，到2002年，这将把超过95%的小鼠基因组序列置于公共领域，供所有人探索。这些序列信息将成为老鼠和人类基因组的基因发现和注释的无价工具。

We plan to accomplish the following goals, as outlined in the application. In the first year: Generate fingerprint data for BAC clones covering the entire mouse genome and assemble contigs. Use 15,000 probes to position the fingerprint contigs onto the mouse physical and genetics maps. Generate 200,000 BAC end sequences. Establish a web site integrating all genetic, physical and contig data to assist in coordinating the overall mouse sequencing effort. In years two and three: Generate working draft sequence for one- half the mouse genome. In years one through three: Generate 115 Mb of finished sequence. Target this finished sequence to regions of interest to the mouse research community. Fingerprint data will be used to assemble contigs from which minimally overlapping clones can be selected. With contributions from other US labs and the Sanger Centre, this should place more than 95 percent of the mouse genome sequence in the public domain by 2002 for all to explore. This sequence information will be an invaluable tool for gene discovery and annotation of the mouse and human genomes.