Regulation of Human Amino Acid Transport System A Gene

描述(由候选人提供) 欧恩·纳姆博士是约翰·霍普金斯大学医学院的肾病研究员。 他将于2002年7月毕业后被任命为初级教员。 他的肾脏科奖学金训练的临床部分。的最终目标是 他的职业生涯是发展成为该领域的独立内科医生兼科学家 ，并对肾脏疾病进行前沿的分子研究。 应激条件下肾细胞存活的分子机制。这个 约翰霍普金斯大学的肾病科将为他的 作为一名内科科学家的职业。在为这个目标做准备的过程中，纳姆博士 在他的肾脏科研究员生涯的头三年里，他在 H.Moo Kuan博士研究肾细胞的分子机制 在高渗应激下存活。最近，他发现了氨基酸运输 系统A2(ATA2)mRNA在培养的肾细胞中显著上调 以及在急性高渗应激时动物的肾脏和脑中。他 计划探索ATA2基因是如何被 细胞外紧张度的变化。此外，他还将勾勒出 ATA2激活在急性高渗应激时细胞存活中的作用。其结果是 这项研究将导致一种新的分子途径的发现 这是高渗应激过程中细胞早期适应的基础。这项研究将 也有助于更好地理解大脑的适应机制 细胞在血清渗透压的病理变化过程中，这是必不可少的 高钠血症或低钠血症患者的处理。在整个资助过程中 在此期间，他将在H·武权博士的精心监督下，他是 转录领域最杰出的科学家之一 亲和性渗透调节基因的调控。纳姆？S博士的研究也将 由桑德拉·E·古吉诺博士密切指导，他开创了循环 肾和肠上皮细胞的核苷酸依赖性阳离子通道 细胞。除了实验室研究外，拟议的计划还旨在 包括参加最先进生物医学研讨会和分子 生物讲座，为他提供全面的分子生物学和 科学的方法。在颁奖期结束时，纳姆博士将获得 成为一名独立调查员的专业知识，并有能力申请 用于独立开展研究的拨款。

DESCRIPTION (provided by candidate) Dr. Ohnn Nahm is a nephrology fellow at the Johns Hopkins School of Medicine and will be appointed as a junior faculty member in July 2002 after completing the clinical part of his nephrology fellowship training. The ultimate goal for his career is to develop into an independent physician-scientist in the field of renal medicine, and to conduct cutting edge molecular research on the molecular mechanism of kidney cell survival under stressful conditions. The nephrology division at Johns Hopkins will provide an ideal environment for his career as a physician-scientist. In preparation for this goal, Dr. Nahm has spent the first three years of his nephrology fellowship in the laboratory of Dr. H. Moo Kwon studying molecular mechanisms responsible for renal cell survival under hypertonic stress. Recently, he discovered amino acid transport system A 2 (ATA2) mRNA to be significantly upregulated in cultured renal cells as well as in kidney and brain of animals during acute hypertonic stress. He plans to pursue the molecular mechanism of how ATA2 gene is regulated by changes in extracellular tonicity. In addition, he will delineate the role of ATA2 activation in cell survival during acute hypertonic stress. The outcome of this study will lead to the discovery of a novel molecular pathway that underlies the early cell adaptation during hypertonic stress. This study will also contribute to a better understanding on the adaptive mechanism of brain cells during pathologic changes in serum osmolality, which is essential for management of patients with hyper-or hyponatremia. During the entire funding period, he will be under the meticulous supervision of Dr. H. Moo Kwon, who is one of the most distinguished scientists in the field of transcriptional regulation of compatible osmolyte genes. Dr. Nahm?s research will also be closely guided by Dr. Sandra E. Guggino, who has pioneered cyclic nucleotide-dependent cationic channels in renal and intestinal epithelial cells. In addition to laboratory research, the proposed program is designed to include attendance at state-of-the-art biomedical seminars and molecular biology lectures to provide him a thorough training in molecular biology and scientific methods. At the end of the award period, Dr. Nahm will have acquired the expertise to become an independent investigator with the means to apply for grants to independently conduct research.