Modelling, design and evaluation of a new in-situ, real time, affinity-based biosensor for monitoring release of stress markers from Chinese Hamster

目前，用于优化蛋白质合成的在线监测，包括测量细胞质量或环境参数，无法提供与细胞生理完整性相关的信息。对细胞质量标记物进行原位监测的优点是，这些变化将允许预测关键现象，例如细胞的生理退化。该项目旨在研究一种新的生物传感方式，用于实时监测细胞内生物标志物，作为生物反应器内细胞健康的指标。它将涉及同步，集成开发纳米晶体，多孔3D生物传感器，具有强大的，生物相容的封装，并量化生物反应器输入对这些测量细胞质量参数的影响。长期目标是对生物反应器内存在的生物过程有更深入的了解，从而提高生物制造的质量控制和可靠性。

Currently, online monitoring used to optimise protein synthesis, involving either measurement of cell mass or environmental parameters, fail to provide information relating to the physiological integrity of the cells. In-situ monitoring of cell-quality markers has the advantage that changes will allow critical phenomena, such as physiological deterioration of the cell, to be predicted.This project aims to investigate a new bio-sensing modality for real-time monitoring of intracellular biomarkers, as indicators of cell health within a bioreactor. It will involve concurrent, integrated development of a nanocrystalline, porous 3D biosensor, with robust, biocompatible encapsulation, and quantify the impact of bioreactor inputs on these measured cell-quality parameters.The longer term goal is to arrive at a deeper understanding of the biological processes that exist within a bioreactor, and thereby enhance quality control and reliability in bio-manufacturing.