Development of enzymes to enhance laundry detergent product performance

该项目旨在在家用清洁产品中引入新型酶，以提高去污性能。用酶改善衣物洗涤剂和餐具洗涤剂的去污性能不仅将提高消费者满意度，而且还将减少相关的环境负担。酶可以取代更多的有毒成分;减少衣物重复洗涤的需要;并使用短的冷水洗涤周期实现高标准的清洁。该项目将重点开发和应用新型氧化还原酶和多糖降解酶，以解决目前酶不针对的底物和污渍。目前洗衣粉中的漂白技术是基于过硼酸盐和过碳酸盐，这些技术不能融入液体洗衣粉中，在低温下不能有效工作，并导致水生富营养化。氧化还原酶为漂白污渍提供酶解。类胡萝卜素污渍，如番茄酱和意大利酱料，是高发病率的污渍，仍然不能很好地消除今天的现有洗涤剂。本项目将鉴定和鉴定新型的类胡萝卜素降解酶，可以有效地漂白类胡萝卜素污渍。多糖天然存在于食品中，也用作个人护理和食品的添加剂，它们是粘性物质，可以增加织物和陶瓷的污垢粘附力。通过确定与污垢粘附有关的特定多糖，可以识别针对这些坚韧污渍的酶并将其应用于洗衣洗涤剂，从而更有效地去除污渍。这些多糖降解酶的性能将遵循基于分子探针的新方法。

The project aims to introduce new classes of enzymes in household cleaning products to improve the stain removal performance. Improving the stain removal performance of laundry and dishwashing detergents with enzymes will not only improve consumer satisfaction, but will also decrease the associated environmental burden. Enzymes can replace more toxic ingredients; reduce the need for clothes to be rewashed; and achieve high standards of cleaning using short, cold washing cycles. The project will focus on the development and the application of novel oxidoreductases and polysaccharide-degrading enzymes, which address substrates and stains not currently targeted by enzymes.Current bleaching technology in laundry detergent is based on perborate and percarbonate, these technologies cannot be incorporated into liquid laundry detergent, do not work efficiently at low temperatures, and contribute to aquatic eutrophication. Oxidoreductase enzymes offer an enzymatic solution to bleaching stains. Carotenoid stains, such as tomato ketchup and spaghetti sauce, are high incidence stains that are still not well removed by today's current detergents. This project will identify and characterise novel carotenoid degrading enzymes that can efficiently bleach carotenoid stains.Polysaccharides are naturally present in food and also used as additives in personal care and food products, they are sticky substances that increase soil adhesion to fabrics and ceramics. By determining the specific polysaccharides implicated in soil adhesion, enzymes that target these tough stains can be identified and applied to laundry detergents, leading to more efficient stain removal. The performance of these polysaccharide degrading enzymes will be followed by novel methodologies based on molecular probes.