Multiplexed Analysis of Protein Tyrosine Kinases

描述(由申请人提供)：这项提议的长期目标是开发一种先进的工具，允许同时和超灵敏地分析多种蛋白激酶抗原。癌症研究和临床实验室迫切需要这样的平台。对在乳腺癌等多种癌细胞中表达或共表达的蛋白酪氨酸激酶(PTK)的多重分析必将为癌症研究和药物开发提供更直接的工具。SuperArray公司开发的ID-Tag蛋白质阵列(IDPA)技术可用于PTK多路分析。这项技术基于设计多样化和独特的ID标签，将悬浮阵列中的蛋白质-蛋白质相互作用信号转换为能够在DNA阵列上被检测和解码的先进技术，该技术是由SuperArray Inc.为DNA微阵列开发的。

DESCRIPTION (provided by applicant): The long-term goal of this proposal is to develop an advance tool that allows simultaneous and ultrasensitive analysis of multiple protein kinase antigens. Such platform is urgently needed in cancer research and clinical laboratories. Multiplex analysis of protein tyrosine kinases (PTKs) expressing or co-expressing in many of the cancer cells such as breast cancers will certainly provide much more straightforward tool for cancer research as well as drug discovery. The ID-Tag Protein Array (IDPA) technology developed in SuperArray Inc. can be used for the PTK multiplexed assays. This technology is based on design of diverse and unique ID-Tags to convert the protein-protein interaction signals in suspension array to be able to be detected and decoded on a DNA array by the advance technology developed in SuperArray Inc. for DNA microarrays.