Higher throughput comet assay for genotoxicity studies

描述（由申请人提供）：彗星分析允许在任何可接近的细胞群中检测DNA损伤，并且通过对基本分析的调整，可以对DNA损伤进行分子定义。Trevigen开发了一个彗星分析平台，由一个经过特殊处理的玻璃支架组成，促进琼脂糖的粘附，并划定了样品应用区域。本第一阶段提案描述了高通量彗星分析格式的开发和验证。该系统将包括一个多样品应用区域的支持，用于标准显微镜平台和当前数据分析软件包的直接样品应用。原型将在标准和改进的彗星试验中进行验证。长期目标是提供一种快速、廉价、标准化和有效的检测方法，用于监测药物或其他治疗的遗传毒性。该方法既可以应用于培养细胞，在筛选过程的早期评估药物，也可以应用于从动物组织中获得的样本，用于药物代谢物的特定器官毒性监测。

DESCRIPTION (provided by applicant): The comet assay allows for the detection of DNA damage in any accessible cell population and a molecular definition of the DNA damage is possible with adaptation of the basic assay. Trevigen has developed a platform for the comet assay consisting of a specially treated glass support, promoting the adherence of agarose, with demarcated sample application areas. This phase I proposal describes the development and validation of a higher-throughput format for comet assay. The system will consist of a support with multisample application areas pretreated for direct sample application for use with standard microscope platforms and current data analysis packages. The prototype will be validated for both the standard and the modified comet assay. The long term objective is to provide a rapid, cheap, standardized, and validated assay for the monitoring of genotoxicity of drugs or other treatments. The assay can be applied to both cells in culture for assessment of drugs very early in the screening process, and to samples obtained from animal tissue for specific organ toxicity monitoring of drug metabolites.