Single DNA Sequencing with Tunneling Spectroscopy

描述(申请人提供)：这项研究将创造一种新技术， 能够对RNA、DNA和其他单分子进行直接测序 寡核苷酸。将超灵敏量子点与扫描探针相结合 显微镜下，分子拓扑与化学物质同时记录 以亚纳米分辨率合成。这使得能够确定 核苷序列及单核苷酸的鉴定 链的多态、扭结、错位和其他突变。这个 原型和开发的软件将实现高吞吐量和自动化 样本分析。 这一新工具将使基于遗传的实现成为可能 医疗保健(药物基因组学)。通过提供一种直接的、非PCR的方法 基因测序，这种信息在基因中的常规应用 倾向筛查、诊断和处方可用于 临床实验室和医院。表达和突变研究还可以 应用于药物开发以及更有效的临床试验 候选人选拔。

DESCRIPTION (provided by applicant): This research will create a new technique, capable of direct sequencing of single molecules of RNA, DNA and other oligonucleotides. By combining ultra sensitive quantum dots with scanning probe microscopy, molecular topology is recorded simultaneously with chemical composition at sub-nanometer resolution. This enables determination of the nucleoside sequence as well as the identification of single nucleotide polymorphisms, kinks, dislocations, and other mutations of the strand. The prototype and developed software will allow high throughput and automated sample analysis. This novel tool will be enabling for the realization of genetic-based healthcare (pharmacogenomics). By providing a direct, non-PCR method for genetic sequencing, routine application of this information to genetic predisposition screening, diagnosis, and prescription becomes available for clinical laboratories and hospitals. Expression and mutation studies can also be applied to drug development, as well as for more effective clinical trial candidate selection.