Determination of the changes in the regulation of urothelium signalling in the ageing bladder

膀胱疾病的患病率随着年龄的增长而显著增加，65岁以上的成年人中有三分之一患有尿失禁。虽然尿失禁不会危及生命，但它往往会导致严重的焦虑，社会孤立和尊严的丧失。正常膀胱功能的调节是通过从膀胱的神经和衬里（尿道鞘）释放的递质的复杂相互作用。我们的假设是，尿路刺激信号机制随着年龄的增长而减少，导致膀胱收缩力调节的丧失，表现为年龄相关的膀胱功能障碍。我们将开发新的灵活的传感器生物传感器，可用于测量收缩性研究过程中的尿激素递质。我们将进行生化分析，以进一步阐明机制，其中的水平和关键酶的表达和分子目标的递质将被测量。这些研究将被转化为老化的人类膀胱。

The prevalence of bladder diseases significantly increases with age, with one third of adults over 65 suffering from urinary incontinence. Although incontinence is not life threatening, it can often lead to significant anxiety, social isolation and a loss of dignity. Regulation of normal bladder function is through complex interplay of transmitters released from the nerves and lining (urothelium) of the bladder. Our hypothesis is that urothelium signalling mechanisms are reduced with age, leading to a loss in the regulation of bladder contractility that presents as age-related bladder dysfunction. We will develop novel flexible sensor biosensors that can be used to measure urothelium transmitters during contractility studies. We will conduct biochemical assays to further characterise the mechanism, where the level and expression of key enzymes and molecular targets of transmitters will be measured. These studies will be translated to characterise the ageing human bladder.