REGULATION OF FUNCTIONAL RECOVERY AFTER SPINAL INJURY

描述：（逐字从申请人的摘要）幼虫七鳃鳗 在脊髓损伤后表现出轴突再生和功能恢复 病变然而，PI发现，通过改变温度， 动物被维持，有可能产生动物， 功能或功能失调的游泳行为。因此七鳃鳗提供了 有机会研究功能失调再生的性质和后果。 她进一步发现了再生系统之间的解剖学差异 在这两个条件下，具体来说， 肾上腺素能轴突。横切后， 5-羟色胺纤维在该地区的吻病变，和一个戏剧性的损失，在尾部 片段然而，在有利于恢复的条件下，血清素纤维 在尾段的含量更高她假设血清素纤维 至少造成了一种行为差异， 不同的温度条件。 PI现在建议确定血清素增加的来源 在吻段的神经支配，和在尾段丢失的5-羟色胺的命运， 片段一种可能性，将探讨的是，增加尾部 节是由于collareral发芽下降血清素系统。她 我将通过评估血清素纤维是否起源于 从脑干或从背根。多巴胺纤维的命运也将 被评价。其他研究将评估的功能能力， 动物使用复杂的随机相位模型来评估生理 应答这项技术是由PI开发的，它提供了一个 对各部分之间协调程度的定量测量。在 同样的动物，再生纤维将被追踪解剖和使用 GABA和甘氨酸的特异性标记

DESCRIPTION: (Verbatim from the Applicant's Abstract) The larval lamprey exhibits axonal regeneration and functional recovery following spinal cord lesions. However, the PI has found that by altering the temperature at which animals are maintained, it is possible to produce animals that exhibit either functional or dysfunctional swimming behavior. Thus the lamprey offers the opportunity to study the nature and consequences of dysfunctional regeneration. She has further found anatomical differences between the regenerated systems under the two conditions, specifically in terms of the distribution of serotoninergic axons. Following transections, there is a dramatic increase in serotonin fibers in areas rostral to the lesion, and a dramatic loss in caudal segments. However under conditions that favor recovery, serotonin fibers are more abundant in the caudal segments. She hypothesizes that serotonin fibers are responsible for at least one form of the behavioral differences seen under the different temperature conditions. The PI now proposes to identify the sources of the increased serotonin innervation in rostral segments, and the fate of the lost serotonin in caudal segments. One possibility that will be explored is that the increases in caudal segments is due to collareral sprouting of descending serotonin systems. She will test this hypothesis by evaluating whether the serotonin fibers originate from the brainstem or from dorsal roots. The fate of dopamine fibers will also be evaluated. Other studies will evaluate the functional capabilities of the animals using a sophisticated stochastic phase model to evaluate physiological responses. This technique, which has been developed by the PI, provides a quantitative measure of the degree of coordination between segments. In the same animals, the regenerated fibers will be traced anatomically and using specific markers for GABA and glycine.