Multispectral histology - Characterisation of tissues at a cellular and molecular level

主要研究目标：开发计算和实验方法，用于开发一种“逐个细胞”的方法来可视化和解释来自质谱学和质谱学成像实验的结果。项目计划：第一年：细胞的MS和MSI-细胞数量的优化和纯培养颗粒的制备。在基质辅助激光解吸/电离(MALDI)和解吸电喷雾电离(DESI)微卫星技术以及快速蒸发电离质谱仪(REIMS)方面的培训。数据分析和可视化方法方面的培训。从纯样本和混合样本中生成光谱。第2年：为在线FACS MS开发FACS MS源。回顾从混合人群中解释数据的多光谱“光谱分解”方法。第3年：仪器和协议的继续发展。第4年：数据分析，分类方法的优化。传播。

Key Research Objective: To develop computational and experimental methods which will be used to develop a 'cell by cell' approach to visualising and interpreting results from mass spectrometry and mass spectrometry imaging experiments.Project Plan:Year 1: MS and MSI of cells - optimisation of cell numbers and preparation of pellets form pure cultures. Training in matrix-assisted laser desorption/ionization (MALDI) and Desorption electrospray ionization (DESI) MSI and in Rapid Evaporative Ionization Mass Spectrometry (REIMS). Training in data analysis and visualisation methods. Generate spectra from pure and mixed samples.Year 2: Development of FACS MS source for an online FACS MS. Review of multispectral 'spectra unmixing' approaches for interpretation of data from mixed populations.Year 3: Continued development of instrumentation and protocols.Year 4: Data analysis, optimisation of classification approaches. Dissemination.