Role of intestinal SIRT1 during liver regeneration

肝脏是一个自我更新的器官，它通过一个迷人而高度复杂的过程--肝脏再生--在体内平衡期间保持其生理活动。这一过程还允许肝脏在受到挑战(用药物/毒素)或手术切除后完全恢复其质量和功能，啮齿类动物在10天内，人类在6-8周内。肝脏是人体的主要代谢器官，在此合成必需的代谢物，为全身提供葡萄糖、脂肪、蛋白质和胆汁酸。肝脏不能单独做到这一点，需要肠道从我们所吃的食物中提取营养，为肝脏的新陈代谢活动提供所需的构件。不幸的是，我们的身体面临着持续不断的挑战，其中一些挑战涉及肝脏功能的丧失，而肝脏需要在不利的条件下再生。为了更好地了解肝脏是如何再生的，我们将能够提出保存和改善肝脏代谢功能以及整体身体健康的策略。该项目的目的是确定支持肝脏再生的机制，更具体地说，确定特定的肠道代谢调节因子在这一过程中的作用。将学习活体技术，包括动物处理和复杂的外科手术，以及为体外研究分离原代细胞。将进行细胞生物学方法方面的培训，包括用定量聚合酶链式反应分析核酸和用蛋白质印迹、酶联免疫吸附试验和免疫组织化学分析蛋白质。还将学习如何通过流式细胞仪来描述免疫反应。

The liver is a self-renewing organ that maintains its physiological activity during homeostasis via a fascinating and highly complex process named liver regeneration. This process also allows the liver to fully restore its mass and function after being challenged (with drugs/toxins) or surgically resected within 10 days in rodents and 6-8 weeks in humans. The liver is the main metabolic organ of the body where the synthesis of essential metabolites occurs, feeding the whole body with glucose, lipids, proteins and bile acids. The liver cannot do this alone and needs the intestine to extract nutrients from the food we eat, providing the liver with the building blocks needed for its metabolic activity. Unfortunately, our body faces constant challenges, some involving the loss of function of the liver that needs to regenerate in unfavourable conditions. To gain a better understanding of how the liver regenerates will enable us to propose strategies to preserve and improve liver metabolic function and overall whole-body health. The aim of this project is to define the mechanisms underpinning liver regeneration and more specifically, to determine the role of specific intestinal metabolic regulators in this process. In vivo techniques will be learned including animal handling and complex surgical procedures, as well as isolation of primary cells for in vitro studies. There will be training in cellular biology methodologies including, analysis of nucleic acids by qPCR and proteins by western blot, ELISA and immunohistochemistry. How to characterise the immune response by FACS will also be learned.