MECHANISIMS OF SYNUCLEIN PATHOGENESIS IN MSA

神经细胞或神经胶质中丝状聚集体的异常积聚是神经病理学的

Abnormal accumulations of filamentous aggregates in neurons or glia are neuropathological hallmarks of many sporadic and hereditary neurodegenerative diseases. For example, Lewy bodies (LBs) formed by filamentous alpha-synuclein (a-syn) aggregates in neurons are characteristic of Parkinson's disease (PD), dementia with LBs (DLB) and an Alzheimer's disease (AD) subtype known as the LB variant of AD (LBVAD), while glial cytoplasmic inclusions (GCIs) composed of a-syn filaments are signature lesions of multiple system atrophy (MSA). The hypothesis that GCIs and LBs play a role in neurodegenerative disease was viewed with skepticism until it was shown that mutations in the a-syn gene cause familial PD, that a-syn is a major component of LBs and GCIs, and that mutant and wild type a-syn form filaments in vitro similar to those in LBs and GCIs. Moreover, it also was shown that beta- (b-syn) and gamma-synucleins (g-syn) accumulate in dystrophic processes, and that a-syn is selectively nitrated in GCIs of MSA as well as in a-syn lesions of all synucleinopathies. Thus, we hypothesize that the accumulations of filamentous a-syn aggregates are induced in part by oxidative/nitrative damage that plays an important role in the onset/progression of MSA. To test this hypothesis specifically in MSA, Aims 1-4 of this Project will analyze the potential contribution of oxidative/nitrative damage in a-syn filament formation and a-syn fibril aggregation into GCIs. In addition, Aims 5 and 6 will determine if/how GCIs compromise the viability of affected glial cells. Insights into the underlying mechanisms of synuclein pathologies and how they contribute to brain degeneration may lead to improved strategies for the diagnosis and treatment of MSA.