Core--Analytical glycotechnology

描述（由申请人提供）： 分析糖技术核心的目的是提供技术专长， 结构表征N-和O-连接唾液酸所需的仪器 从来自以下的蛋白质、细胞或组织分离的寡糖或糖胺聚糖： 转基因动物由项目负责人实验室提供。核心将大大受益 从获得现有糖化技术中的仪器、试剂和专业知识， 加州大学圣地亚哥分校糖生物学研究和培训中心（GRTC）的资源核心。 这些分析所采用的许多技术（脂质提取、聚糖分离、单糖分析、甲基化连接分析、糖胺聚糖二糖分析）是在上一个供资期间设计或实施的。其他的只是最近才成为可用的GRTC核心增加了新的，国家的最先进的设备，旨在提高灵敏度。这些升级包括用于现有LC-Q质谱仪的纳米喷雾源，MALDI-TOF质谱仪，高灵敏度荧光检测器和用于NMR的新纳米探针。高质量仪器的可用性，加上多年的分析糖生物学经验， GRTC糖技术资源，使我们能够满足所提出的分析挑战， 项目负责人和执行必要的分析，以支持他们的研究目标。

DESCRIPTION (provided by applicant): The purpose of the Analytical Glycotechnology Core is to provide the technical expertise and instrumentation necessary to structurally characterize sialic acids,N- and O-linked oligosaccharides or glycosaminoglycans isolated from either proteins, cells or tissues from transgenic animals provided by the Project Leaders' laboratories. The Core will greatly benefit from access to the instruments, reagents and expertise in the existing Glycotechnology Resource Core of the UCSD Glycobiology Research and Training Center (GRTC). Many of the techniques to be employed for these analyses (lipid extraction, isolation of glycans, monosaccharide analysis, methylation linkage analysis, glycosaminoglycan disaccharide analysis) were devised or implemented during the previous funding period. Others have only recently become available as the GRTC Core has added new, state-of-the-art equipment geared toward improving sensitivity. These upgrades include a nanospray source for the existing LC-Q mass spectrometer, a MALDI-TOF mass spectrometry, a high sensitivity fluorescence detector, and a new nanoprobe for the NMR. The availability of high quality instrumentation, coupled with the many combined years of analytical glycobiology experience represented by the members of the GRTC Glycotechnology Resource, allow us to meet the analytical challenges posed the Project Leaders and perform the analyses necessary to support their research aims.