CALCIUM AND INTEGRATION IN PYRAMIDAL CELLS

描述：(申请人摘要) 该项目的长期目标是促进对 突触整合和可塑性的树突机制。这些 基本的细胞过程在更全球化的 信息处理、学习和记忆的机制。在当前 建议，我们将注意力集中在大鼠CA1区的神经元上 海马体，在脑片制备中研究。 我们感兴趣的是神经调节剂是如何从弥漫纤维中释放出来的 在海马区，影响棘波的传播和相关的[Ca2+]i变化 锥体神经元的树突。我们将分析[Ca2+]i的调制 由于尖峰电压分布的变化而发生的变化，调制 树突状钙通道与细胞内钙释放的调控 商店。我们还将分析尖峰电流的传播和[钙]i的变化 中间神经元。 我们将结合高速荧光技术来分析这些问题 用钙敏感指示剂对单个神经元进行成像，以及 细胞体和树突的全细胞记录。电子录音 在记录现场直接显示有关脉冲传播的信息。 成像数据给出了在不同的不同情况下更全面的尖峰传播图像 神经元的某些部分以及直接揭示关于 活性依赖性[Ca~(2+)]i变化。

DESCRIPTION: (Applicant's Abstract) The long-term goal of this project is to contribute to the understanding of dendritic mechanisms underlying synaptic integration and plasticity. These fundamental cellular processes play an important role in the more global mechanisms of information processing, learning and memory. In the current proposal, we are focusing our attention on neurons in the CA1 region of the rat hippocampus, studied in the brain slice preparation. We are interested in how neuromodulators, normally released from diffuse fibers in the hippocampus, affect spike propagation and associated [Ca2+]i changes in the dendrites of pyramidal neurons. We will analyze the modulation of [Ca2+]i changes due to changes in the voltage profile of the spikes, modulation of dendritic Ca2+ channels, and control of the release of Ca2+ from internal stores. We also will analyze spike propagation and [Ca2+]i changes in interneurons. We will analyze these problems using a combination of high-speed fluorescence imaging of individual neurons filled with Ca2+-sensitive indicators, and whole-cell recording from cell bodies and dendrites. The electrical recordings directly reveal information about spike propagation at the site of recording. The imaging data give a more global picture of spike propagation in different parts of the neuron as well as directly revealing information about activity-dependent [Ca2+]i changes.