MESSENGER RNA DECAY OF IMMEDIATE EARLY GENES

徐博士的这一建议是他从1990年开始工作的延续。 上一个融资期。他取得了重大进展和贡献， ARE介导的mRNA周转的理解，似乎有所有的 继续这样做的基础。该提案调查了hnRNP D的作用 (also已知为AUF 1）在含ARE的mRNA的降解中以及如何 翻译后修饰和相关蛋白质影响这一点 活动下面的具体目标是为了获得问题的答案， 以下问题：1.压力激活信号在其中扮演了什么角色 途径和泛素-蛋白酶体途径在ARE指导的mRNA周转？2. ARE依赖性mRNA所必需的ARE/蛋白质衰变复合物是什么 营业额？3. hnRNP D的哪些结构特征对于其作为 在ARE介导的mRNA在体内衰变的不稳定蛋白？4.如何在ARE 发挥其破坏稳定的功能？调查员建议解决这一问题 问题通过采用体外RNA衰变系统来剖析和表征 靶向ARE的mRNA衰减。

This proposal by Dr. Shyu is a continuation of his work from the previous funding period. He has made significant progress and contributions to the understanding of ARE-mediated mRNA turnover and appears to have all the groundwork to continue to do so. The proposal investigates the role of hnRNP D (also known as AUF1) in the degradation of ARE-containing mRNAs and how post-translational modifications and associated proteins influence this activity. The specific aims below are designed to obtain answers to the following questions: 1. What are the roles played by stress-activated signaling pathways and the ubiquitin-proteasome pathway in ARE-directed mRNA turnover? 2. What is the ARE/protein decay complex necessary for ARE-dependent mRNA turnover? 3. What structural features of hnRNP D are necessary for its role as a destabilizing protein in ARE-mediated mRNA decay in vivo? 4. How does the ARE exert its destabilizing function? The investigator proposes to address this question by employing in vitro RNA decay systems to dissect and characterize the ARE-targeted mRNA decay.