PURCHASE OF A VARIAN INOVA 600 MHz NMR SPECTROMETER

我们建议为斯坦福大学化学系购买一台600兆赫瓦里安Inova核磁共振光谱仪。该仪器将使研究人员能够确定大分子的高精度结构，包括蛋白质、核酸、蛋白质-蛋白质、蛋白质-配体和蛋白质-核酸复合物。该仪器将直接支持pi和co- pi的研究小组。s（代表大约80名研究人员），预计他们将是主要用户，但也将提供给整个部（大约350名研究人员）。仪器将放置在化学系核磁共振实验室的现有空间，由2名全职核磁共振博士和5名研究生助理维护。提出的研究特别确定需要一个专门的高场核磁共振仪器用于生物分子结构分析。这些研究包括合成天然产物抗生素的酶的结构研究，抗癌药物及其与靶酶的相互作用，参与细胞凋亡的聚酮及其衍生物，参与免疫反应的蛋白质-蛋白质和蛋白质-配体相互作用，参与DNA复制和DNA修复的DNA螺旋，以及与丙型肝炎病毒有关的RNA。所有私家侦探的研究项目目前都得到了美国国立卫生研究院的支持，大部分也得到了美国国家科学基金会的支持。在化学系现有的设备上，这项工作几乎无法完成，而且所有四名私家侦探最近都被迫到其他地方进行必要的结构研究，这阻碍了他们的研究。我们建议购买的具体仪器包包括Oxford 14.1 T磁铁，以及超级shims，两个全频段RF通道，三个总RF通道，每个通道的波形发生器，可变温度单元，脉冲场梯度，Sun工作站和低温探头。

We propose to purchase a 600 MHz Varian Inova Nuclear Magnetic Resonance spectrometer for the Chemistry Department at Stanford University. This instrument will enable researchers to determine high precision structures of macromolecules including proteins, nucleic acids, and protein-protein, protein-ligand, and protein-nucleic complexes. The instrument will directly support the research groups of the P.I. and co-P.I.s (representing approximately 80 researchers), and it is expected that they will be the major users, but will also be available to the Department as a whole (approximately 350 researchers). The instrument will be placed in existing space in the NMR lab in the Chemistry Department, and will be maintained by 2 full-time Ph.D. NMR staffpersons and by 5 graduate assistants. The research proposed specifically identifies the need for a devoted high field NMR instrument for biomolecular structure analysis. Such research includes structural studies of enzymes that synthesize natural product antibiotics, anticancer drugs and the interaction with their target enzymes, a polyketide and derivatives involved in apoptosis, protein-protein and protein-ligand interactions involved in immune response, DNA helices involved in DNA replication and DNA repair, and RNA relating to Hepatitis C virus. All of the P.I.s' research programs are currently supported by NIH and most by NSF as well. Little of this work can be accomplished on current equipment of the Chemistry Department, and all four P.I.s have been forced recently to go elsewhere to carry out needed structural studies, hampering their research. The specific instrumental package we propose to purchase includes the Oxford 14.1 T magnet, as well as supershims, two Fullband RF channels with three total RF channels, waveform generators for each channel, a variable temperature unit, pulsed field gradients, a Sun workstation, and a cryo probe.