Zeiss LSM 510 Laser Scanning Microscope

描述（由申请人提供）：九个高产NIH资助的 研究人员与19个NIH资助的项目（>$3，200，000 ADC）在两个 科罗拉多州立大学的各部门要求341,154美元的NCRR资金， 购买蔡司LSM 510共聚焦激光扫描显微镜， 现已解散，但以前大量使用的10岁的萨拉斯特罗/分子动力学 MultiProbe 2001共聚焦显微镜。在过去的四年里， 解剖学和神经生物学已经花费了超过47，000美元来保留Sarastro 显微镜的功能，但它经常在关键实验中失败， 它的能力是如此有限，以至于它现在只对一个群体有用， 其仅用于获得组织的低分辨率图， 冷冻断裂分析因此，所有主要用户组的成员必须 要么前往其他城市获取数据，要么必须依赖 在其他国家和加拿大的合作者获得可靠的数据。扫描速率 蔡司共焦相机的速度至少比以前快20倍 仪器（156帧/分钟， 512 x512），从而允许分析活细胞中的瞬态现象。 蔡司LSM 510在此应用中配置有三个激光器 （不可分级至四个），自动化和可再现的图像捕获;三个 同时记录的荧光通道（加上可见光）;以及 同步隔行双激光扫描（“多跟踪”， 大多数其他共焦系统）。后一种功能允许图像捕获 足够快，以允许实时分析生物过程， 活细胞（至少6个NIH资助的项目所必需的）。还必须 LSM 510是一款多用户设备，具有强大的设计和直观的成像功能 软件，为"aboutroutine”和“aboutexpert”用户预设模式 （这消除了大多数手动调整）。共聚焦显微镜是必不可少的， 研究钙信号，膜的分子组成， 神经元传递的突触和非突触机制及其相关性 of alterations改变in membrane膜molecular分子composition组成with changes变化in physiological生理 活动和/或疾病状态。蔡司510将安装在数字 成像中心，其运作是100%由该部资助， 解剖学和神经生物学，允许使用设备，每小时不收费（0美元）， 用户.一名半职主任将维护仪器并培训新用户。 DIC董事会将每两个月举行一次会议，以确保持续有效的 调度和操作的更换共聚焦显微镜，考虑 未来的升级，以及建立或更改DIC访问策略。

DESCRIPTION (provided by applicant): Nine highly-productive NIH-funded investigators with nineteen NIH-funded projects (>$3,200,000 ADC) in two departments at Colorado State University request $341,154 in NCRR funds to purchase a Zeiss LSM 510 Confocal Laser Scanning Microscope to replace a now-defunct but previously heavily-used 10-year old Sarastro/Molecular Dynamics MultiProbe 2001 Confocal Microscope. In the past four years, the Department of Anatomy and Neurobiology has spent more than $47,000 to keep the Sarastro microscope functioning, but it has failed so often during critical experiments, and it is of such limited capability that it is now useful to only one group, which uses it solely to obtain low resolution maps of tissues for freeze-fracture analysis. Thus, members of all of the Major Users groups must either travel to other cities to obtain data, or they must rely on collaborators in other states and Canada to obtain publishable data. Scan rates for the Zeiss confocal are at least 20 times faster than the previous instrument (156 frames per minute vs. maximum of 8 frames per minute at 512x512), thereby permitting analysis of transient phenomena in living cells. The Zeiss LSM 510 as configured in this application has three lasers (ungradable to four), automated and reproducible image capture; three fluorescence channels (plus visible light) recorded simultaneously; and simultaneous interlaced dual laser scanning ("multi-tracking," not available on most other confocal systems). The latter capability allows image capture sufficiently rapidly to permit real-time analysis of biological processes in living cells (essential for at least 6 NIH-funded projects). Also important for a mult-iuser facility, the LSM 510 has a robust design and intuitive imaging software, with preset modes for both aboutroutine" and aboutexpert" users (which eliminates most manual adjustments). Confocal microscopy is essential in projects investigating calcium signaling, molecular composition of membranes, synaptic and non-synaptic mechanisms of neuronal transmission, and correlation of alterations in membrane molecularcomposition with changes in physiological activity and/or disease state. The Zeiss 510 will be installed in the Digital Imaging Center, whose operation is 100 percent funded by the Department of Anatomy and Neurobiology, allowing use of equipment at no ($0) hourly charge to users. A half-time Director will maintain the instrument and train new users. The DIC Board of Directors will meet bi-monthly to insure continued efficient scheduling and operation of the replacement confocal microscope, to consider future upgrades, and to establish or change DIC access policies.