OPTIMA XL-I ANALYTICAL ULTRACENTRIFUGE & ACCESSORIES

描述（由申请人提供）：本提案的参与者需要 来自Beckman Coulter的XL-I分析超离心仪以及 各种必要的配件，以推进他们的研究项目。的 参与研究的科学家来自沃兹沃斯中心、奥尔巴尼医学院， 奥尔巴尼药学院。目前，没有分析性的超浓缩物， 这一区域PI对旧型号F有着丰富的经验 超级分析师，还有另外两名参与者。仪器将 位于沃兹沃斯中心现有核心设施内，PI 核心导演PI和副核心总监将拥有 成为这项技术的专家并培训他人 它的使用和应用。由于它将位于现有的核心， 有几个工具，有相当多的专业知识，如何正确地 提供这样的科学服务。一个突出的原因，以获得分析 最重要的是，它将增强其他现有的仪器， 其包括动态光散射、HPLC、圆二色性和 荧光。这些项目主要由NIH资助，涉及研究项目 分析超级计算机能够处理的：包括 准确测定低聚物的状态， 蛋白质，研究不同分子之间的复合物形成，以及形状 分子的变化。一些项目建议研究复杂的 不同的蛋白质或蛋白质和核酸之间的相互作用。的 分析型超临界流体有许多仪器策略， 各种强大的软件协议来解决这些生物学上重要的 问题它是一种通用的，有时是非常合适的技术， 生物化学和生物物理进展的方法。

DESCRIPTION (provided by applicant): The participants in this proposal require an XL-I analytical ultracentrifuge from Beckman Coulter together with an assortment of necessary accessories, to advance their research projects. The scientists involved are from the Wadsworth Center, Albany Medical College, and Albany College of Pharmacy. Currently there is no analytical ultracentrifuge in this region. The PI has extensive previous experience with the old model F analytical ultracentrifuge, as do two other participants. The instrument will be located at the Wadsworth Center in an existing core facility where the PI is core director. The PI and the associate core director will have the responsibility of becoming expert with this technology and training others in its use and applications. Since it is to be located in an existing core that has several instruments, there is considerable expertise in how to properly provide such a scientific service. A salient reason to acquire the analytical ultracentrifuge is that it will enhance the other existing instrumentation, which includes dynamic light scattering, HPLC, circular dichroism and fluorescence. The projects are mostly NIH funded, and involve research projects that the analytical ultracentrifuge is capable of handling: including the accurate determination of oligomeric states, the self-assembly properties of a protein, the study of complex formation between unlike molecules, and shape changes in molecules. Several of the projects propose to study complex interactions between unlike proteins or proteins and nucleic acids. The analytical ultracentrifuge has a number of instrumentation strategies and various powerful software protocols to solve these biologically important problems. It is a versatile and sometimes singularly appropriate technological approach to biochemical and biophysical progress.