BIACORE 3000 Biosensor

描述（由申请人提供）：本提案的目标是 一个BIAcore 3000生物传感器给韦恩州立大学。这个工具， 旨在检测，监测和量化生物分子相互作用，将是一个 重要资产的研究计划的一些NIH资助的 调查人员在这个机构。目前还没有生物传感器可用 在韦恩州立大学和底特律其他地方都没有。 该文书将在各种方面对申请人大有好处， 正在进行的项目包括i.对蛋白质之间相互作用的研究 参与过氧化物酶体生物发生，ii.雌激素受体的定量 (and突变的雌激素受体）-辅调节因子相互作用，iii.一 组织蛋白酶与细胞外基质相互作用的表征 发生在肿瘤细胞上的蛋白水解级联反应中的蛋白质 表面，iv.雌激素受体如何结合的分子描述 特异性蛋白质、DNA和减弱的雌激素配体，v. 芳香烃受体如何与视网膜母细胞瘤肿瘤抑制因子结合 蛋白质并介导细胞周期控制，和vi.的分析 基质金属蛋白酶及其抑制剂，称为TIMPS， 在癌症中。 韦恩州立大学坚定地致力于这一建议。通过 19名研究人员、医学院和大学的贡献 总体而言，已确保以下人员安全： 1.一项供资的长期计划，用于维持和继续运作 BIAcore 3000生物传感器。 2.位于中心位置的核心设施，用于容纳设备。 3.一名受资助的技术人员，将接受操作和计划使用 仪器 BIAcore 3000生物传感器是一种最先进的仪器， 非常可靠的数据。本文概述的计划 最大限度地有效利用这一工具。

DESCRIPTION (provided by the applicant): The goal of this proposal is to bring a BIAcore 3000 biosensor to the Wayne State University. This instrument, designed to detect, monitor, and quantify biomolecular interactions, will be a significant asset to the research programs of a number of NIH-funded investigators at this institution. At present, there is no biosensor available at Wayne State nor anywhere else in metropolitan Detroit. The instrument will be of great benefit to the applicants in a variety of ongoing projects including i. an examination of the interactions among proteins involved in peroxisome biogenesis, ii. a quantification of estrogen receptor (and mutated estrogen receptor)-.coregulator interactions, iii. a characterization of the interactions of cathepsins and extra-cellular matrix proteins in the context of proteolytic cascades which occur on the tumor cell surface, iv. a molecular description of how the estrogen receptor binds specific protein, DNA, and attenuated estrogen ligands, v. an examination of how the aryl hydrocarbon receptor binds the retinoblastoma tumor suppressor protein and mediates cell cycle control, and vi. an analysis of the interactions of matrix metalloproteinases and their inhibitors, called TIMPS, in cancer. Wayne State University is strongly committed to this proposal. Through the contributions of 19 investigators, the School of Medicine, and the University at large, the following have been secured: 1. A funded long-term plan for maintenance and continued operation of the BIAcore 3000 biosensor. 2. A centrally located core facility to house the equipment. 3. A funded technician who will be trained to operate and schedule usage of the instrument. The BIAcore 3000 biosensor is a state-of-the-art instrument that will provide extremely reliable data for years to come. The plan outlined herein will maximize efficient use of this instrument.