Biacore 3000 Biosensor

申请者提供的描述)：我们申请资金购买Biacore 3000，用于大学的生物传感器核心设施。马里兰州(UM)学校 医学博士(SOM)。Biacore使用表面等离子体共振(SPR)检测 分子或其他材料与衍生的金表面的结合。许多 3000的功能使其成为核心设施的理想之选。(I)SPR检测到 各种生物材料，无论是蛋白质，核酸，脂类， 碳水化合物、病毒或细胞。(Ii)各种稳定的表面化学物质 可从Biacore获得，允许分析许多不同类型的 配基。(3)Biacore有4个独立监测的渠道，便利 对照样品和实验样品的同时比较。(四) 信噪比高，反应条件可精确控制 允许可靠地监控绑定。(V)Biacore可准确测量 小分子的结合。(Vi)液压系统可最大限度地减少 使用的样品，便于结合样品的回收。(Vii)该单位是 易于自动化，允许长时间无人值守使用。 Biacore 3000将主要由5名调查人员使用，4名在UM SOM和 一个在密歇根大学生物技术高级研究中心(CARB)。R·布洛赫 首席研究员，将利用Biacore技术研究相互作用 在可兴奋细胞的膜蛋白和细胞骨架蛋白之间。F. 马戈利斯将研究嗅觉标记蛋白与 使用相展示文库鉴定蛋白质。E.Weinman将研究 上皮性Na，H-交换器调节因子的寡聚作用及其机制 与其他调节蛋白、细胞骨架蛋白和运输蛋白结合。J·方德尔 将定量分析辅活化子蛋白与 甲状腺激素依赖型核受体。R.Mariuzza(CARB)，将研究 抗原与抗体和T细胞受体的结合。Mariuzza hs博士 对早期的Biacore模型有丰富的经验；所有其他四个主要用户 在蛋白质-蛋白质结合的研究中使用了SPR技术，有两个 发表了他们的研究结果。 Biacore 3000将安装在密歇根大学索姆分校的生物传感器核心设施中， 将由经验丰富的蛋白质化学家J.Ursitti博士执导 分子生物学家，已经从Biacore接受过BIA基础训练。 该设施将配备一名受过比亚科尔培训的全职技术人员。一个 由Bloch博士、Fondell博士和Mariuzza博士组成的内部咨询委员会 将监督该设施，以确保其顺利运作，公平进入 适用于所有主要用户。新装修的空间已分配给 生理系的设施，以及确保顺利启动的资金 以及由SOM和该计划承诺的持续支持 细胞和分子生物学。我们预计这一设施将提供其 主要用户，最终是SOM和巴尔的摩地区的其他用户， 最先进的生物传感器技术。

DESCRIPTION provided by the applicant): We request funds to purchase a BIAcore 3000 for use in a Biosensor Core Facility at the Univ. of Maryland (UM) School of Medicine (SoM). BIAcore uses surface plasmon resonance (SPR) to detect binding of molecules or other materials to a derivatized gold surface. Many features make the 3000 ideal for a core facility. (i) SPR detects binding of a variety of biological materials, be they protein, nucleic acids, lipids, carbohydrates, viruses or cells. (ii) A variety of stable surface chemistries are available from BIAcore, allowing analysis of many different kinds of ligands. (iii) The BIAcore has 4 independently monitored channels, facilitating simultaneous comparisons of control and experimental samples. (iv) Signal-to-noise ratios are high, and precise control of reaction conditions permits reliable monitoring of binding. (v) The BIAcore can accurately measure binding of small molecules. (vi) The hydraulic system minimizes the volume of samples used and facilitates the recovery of bound sample. (vii) The unit is readily automated, permitting unattended use for extended periods of time. The BIAcore 3000 will be used primarily by 5 investigators, 4 at the UM SoM and one at the UM Center for Advanced Research in Biotechnology (CARB). R. Bloch, principal investigator, will utilize BIAcore technology to study interactions between membrane proteins and cytoskeletal proteins of excitable cells. F. Margolis will examine the interactions of olfactory marker protein with proteins identified using a phase display library. E. Weinman will study oligomerization of the epithelial Na,H-exchanger regulatory factor and its binding to other regulatory, cytoskeletal, and transport proteins. J. Fondell will analyze quantitatively the interactions of coactivator proteins with the thyroid hormone-dependent nuclear receptor. R. Mariuzza (CARB), will study binding of antigen to antibody and to the T-cell receptor. Dr. Mariuzza hs extensive experience with an earlier BIAcore model; all four other major users have used SPR technology in studies of protein-protein binding, and two have published their results. The BIAcore 3000 will be housed in a Biosensor Core Facility at the UM SoM and will be directed by Dr. J. Ursitti, an experienced protein chemist and molecular biologist who has already received BIA basics training from BIAcore. The facility will be staffed by a full-time, BIAcore-trained technician. An Internal Advisory committee consisting of Drs. Bloch, Fondell, and Mariuzza will oversee the facility to ensure its smooth functioning, equitable access for all the major users. Newly renovated space has been allocated for the facility by the Department of Physiology, and funds to ensure a smooth start-up as well as ongoing support have been committed by the SoM and the program in Cell and Molecular Biology. We anticipate that this facility will provide its primary users, and ultimately others at the SoM and in the Baltimore area, with state-of-the-art biosensor technology.