Analysis of Biomolecular Associations

描述（由申请人提供）：配体结合和大分子 协会通常被归类为“可逆协会” 以表示它们对非共价相互作用的依赖性， 溶液平衡本发明公开了一种表面等离子体共振仪器（BIAcore）， 要求加入我们现有的分析超级计算机，以建立 一个共享的设施的定量分析可逆协会。的 申请者的研究兴趣包括广泛的 重要的生物医学问题，包括基因转录的组装 起始和调节复合物，抗HIV药物设计，配体结合和 变构学、免疫学和神经科学。这些仪器将存放在 位置方便用户的实验室，并将访问 整个爱因斯坦研究社区通过实验室的高分子 分析和蛋白质组学。PI和本提案的其他参与者 在组织和管理共享资源方面有良好的业绩记录。 该机构为实验室提供大量支持， 大分子分析和蛋白质组学，包括仪器支持 负责监督现有分析超级计算机使用的专家， 他将负责本授权书中要求的新仪器。

DESCRIPTION (provided by the applicant): Ligand binding and macromolecular associations are generally grouped under the rubric "Reversible Associations" to denote their dependence upon noncovalent interactions that are in dynamic equilibrium in solution. A surface plasmon resonance instrument (BIAcore) is requested to join our existing analytical ultracentrifuge in order to establish a shared facility for the quantitative analysis of reversible associations. The research interests of the application participants encompass a broad range of important biomedical problems including assembly of gene transcription initiation and regulatory complexes, anti-HIV drug design, ligand binding and allostery, immunology and neuroscience. These instruments will be housed in a location convenient to the users' laboratories and will be accessible to the entire Einstein research community through the Laboratory for Macromolecular Analysis & Proteomics. The PI and other participants in this proposal have a proven track record in the organization and administration of shared resources. The institution provides substantial support to the Laboratory for Macromolecular Analysis & Proteomics including support for the instrument specialist who is overseeing use of the existing analytical ultracentrifuge and who will take charge of the new instrument requested in this grant.