CORE--TISSUE CULTURE FACILITY

描述(改编应用)：组织培养/形态学/包埋照片 [原文如此]移植模型核心将在细胞内提供专业知识和服务 培养、肠道异种移植和各种形态技术，包括 原位杂交、免疫组织化学和显微镜(光镜、共聚焦 激光扫描和电子显微镜)。该服务还将提供咨询服务 关于这些技术和相关技术在 有计划的实验，特别是关于使用人体肠道的实验 作为这一实验工具的异种移植将在一定程度上被所有人利用 这些项目。它将成为这些项目的主要研究资源， 提供集中式细胞培养和显微镜设施。随着更多的 个别项目正在使用细胞生物学方法，研究人员将 需要越来越多地使用细胞培养和形态学技术。作为一部分 对于这种重新应用，我们将在形态学方面大大扩展选择。 我们将在共聚焦显微镜方面提供专家建议和服务，使用单个、 双重和三重标签选项，以及定量分析。我们还将 提供包括多个标记的扩展的荧光显微镜能力， 数字摄影和数字图像的定量分析。这些核心也 为个别项目成员提供特定技术方面的培训 他们的实验室和宝贵的智力和技术资源 关于形态学的科学和方法论途径的交流 探头(定量成像等)。

Description (adapted form application): The Tissue Culture/Morphology/Xenograph [sic] Transplant Model Core will provide expertise and services in cell culture, intestinal xenografting and various morphologic techniques, including in situ hybridization, immunohistochemistry and microscopy (light, confocal laser scanning and electron microscopy). The service will also serve to consult individual research projects on use of these and related techniques in the planned experiments, especially with regard to the use of human intestinal xenografts as this experimental tool will be utilized in some capacity by all the projects. It will be a major research resource for the projects by providing a centralized cell culture and microscopy facility. As more of the individual projects are using cell biology approaches, the investigators will require an increasing use of cell culture and morphologic techniques. As part of this re-application, we will expand considerably the options in morphology. We will provide expert advice and service in confocal microscopy, using single, double and triple label options, as well as quantitative analysis. We will also provide an expanded fluorescent microscopy capacity including multiple labels, digital photography and quantitative analysis of digital images. The cores also offer training in specific techniques to members of the individual projects and their laboratories and a valuable resource for intellectual and technical interchange regarding scientific and methodological approaches to morphologic probes (quantitative imaging, etc.).