CORE--MOUSE FACILITY

鼠标核心将在两个部分中支持程序的各个组件 主要方法：1)制备转基因和基因敲除小鼠； 以及，b)各种突变小鼠系的维护 建议进行的研究。核心的一个主要初始焦点将与 蛋白质4.1基因敲除小鼠和表达不同蛋白的小鼠的建立 蛋白质4.1转基因。建议进行的活体解剖研究 蛋白质4.1的生物学功能在项目1、2、3、 6.核心将在协助参与者方面发挥核心作用 程序设计的各个方面涉及到的小鼠 学习。创造转基因和基因的所有技术步骤 敲除小鼠(即创建各种DNA结构并执行 胚胎操作)将作为这一核心的一部分进行。这个 CORE还将维持和提供地中海贫血小鼠。

The mouse core will support the various components of the program in two major ways: a) the preparation of transgenic and gene knockout mice; and, b) the maintenance of various mutant mouse lines to be used in the proposed studies. A major initial focus of the core will relate to the creation of both protein 4.1 knockout mice and mice expressing various protein 4.1 transgenes. The proposed studies to dissect in vivo the biological functions of protein 4.1 are described in projects 1, 2, 3, and 6. The core will play a central role in assisting the participating programs in designing the various aspects involved in of the murine studies. All the technical steps in creating the transgenic and gene knock- out mice (i.e. creating the various DNA constructs and performing embryo manipulations) will be carried out as part of this core. The core will also maintain and provide thalassemic mice.