Engineering recombinant bacteriophages as multi - tools against intracellular bacterial infections

噬菌体是一种具有高度特异性的病毒，可以感染其细菌宿主，使其成为靶向抗菌剂。目前合成生物学的进展使噬菌体的基因工程得到了广泛的应用。在这个项目中，我们建议对噬菌体进行基因工程，使其能够有效地进入人类细胞，从而允许噬菌体介导的杀灭难以治疗的细胞内大肠杆菌K1感染。遗传学、微生物学、噬菌体生物学、合成生物学、人类细胞生物学和显微镜方法的结合，将使我们能够实现该项目的目标。

Bacteriophages are viruses with great specificity for infecting their bacterial hosts, allowing their use as targeted antibacterial agents. Current advances in synthetic biology have enabled the genetic engineering of bacteriophages for a broad range of applications. In this project, we propose to genetically engineer bacteriophage to efficiently enter into human cells to allow for phage-mediated killing of difficult to treat intracellular Escherichia coli K1 infection. A combination of genetics, microbiology, phage biology, synthetic biology, human cell biology and microscopy methods, will enable us to reach the objectives of the project.