CORE--CELL IMMORTALIZATION AND TRANSFECTION FACILITY

Gero细胞永生化和转基因设施，或称CIT， 将由麦克杜格尔博士作为项目负责人进行监督。服务核心是 旨在为研究核心的4个科学项目服务，每个项目 该项目将需要牙齿特定的和稳定转染的细胞系。这个 Service Core将通过将基因转移到几个 通过使用逆转录病毒癌基因，单层培养中的牙齿细胞类型 构造。将分离稳定的细胞系，并进行基因表达谱和 将对细胞外基质的性质进行分析。这些细胞系将作为 研究核心项目中体外启动子研究的资源。这个 Service Core还将通过各种不同的方式进行稳定的细胞转染 由单个项目提供的发起人-记者构造。

The GERO Cell Immortalization and Transfection Facility, or CIT, will be supervised by Dr. MacDougall as project leader. The Service Core is designed to serve the 4 scientific projects of the Research Core, and each project will require tooth specific and stably transfected cell lines. The Service Core will produce immortalized cell lines by gene transfer to several dental cell types in monolayer culture, through use of retroviral oncogene constructs. Stable cell lines will be isolated and gene expression profile and nature of extracellular matrix will be analyzed. These cell lines will serve as a resource for in vitro promoter studies in the Research Core projects. The Service Core will also perform stable cell transfections with various promoter-reporter constructs provided by the individual projects.