CORE--CELL SCIENCE

细胞科学核心(CSC)是U54中心的组成部分， 为其研究人员和生殖生物学提供服务 由NICHD RBS资助的弗吉尼亚大学的研究人员 布兰奇。CSC的主要目标是完成依赖于 抗原分子和信使RNA的组织和细胞定位 (M RNA)，以及病理组织和细胞的形态分析 改变。肯尼斯·董博士，人类和实验病理学家， 生殖和免疫学方面的专业知识将继续指导CSC。 他将咨询或合作研究需要准备高级 适用于组织学、免疫组织学、 免疫细胞学、原位杂交和凋亡细胞检测，AS 以及抗原分子的组织和细胞定位 动物组织的组织病理学解释。他将与马多夫博士合作。 马克·斯托勒(Mark Stoler)，联席主任、病理学家和原位专家 谁将就需要mRNA的研究提供咨询或合作 检测和定量。他们将组织、确定优先顺序并提高质量- 控制梅丽莎·贝瓦德女士的工作，她是一位经验丰富的历史学家。 玛丽·麦坎纳，一位原位杂交技术专家。在适当的时候， 他们将把技术转移到调查人员的实验室 通过对研究人员、技术人员的培训和对其 工作。CSC还将开发新技术，以响应和 对将在U54中发展的研究需求的预期。示例 包括：1)双抗体标记技术和双抗体/原位 标记技术，2)共聚焦显微镜，3)试剂的获取和 人类翻译和小鼠繁殖研究的技术。 已聘请外部顾问为CSC服务，以支持这一点 努力吧。核心将进行调整，以满足不断变化的研究需求 新的U54上下文。P30-CSC装置已经成功地提供了质量 为越来越多的生殖生物学研究人员提供服务， 负责完成50多项研究项目， 获得了许多NIH的拨款建议。

The Cell Science Core (CSC) is an integral part of the U54 Center, providing services to its investigators and to the reproductive biology researchers at the University of Virginia by funded by the NICHD RBS Branch. The main goal of the CSC is to accomplish projects that rely on tissue and cellular localization of antigenic molecules and messenger RNA (mRNA), as well as morphologic analysis of pathologic tissue and cellular changes. Dr. Kenneth Tung, a human and experimental pathologist with expertise in reproduction and immunology, will continue to direct the CSC. He will consult or collaborate on studies requiring preparation of high quality tissue sections suitable for histology, immunohistology, immunocytology, in situ hybridization and apoptotic cell detection, as well as tissue and cellular localization of antigenic molecules and interpretation of histopathology of animal tissues. He will work with Dr. Mark Stoler, Co-director, a pathologist and an expert in situ hybridization who will consult or collaborate on studies requiring mRNA detection and quantitation. They will organize, prioritize, and quality- control the work of Ms. Melissa Bevard, an experienced histologist and Ms. Mary McCanna, an in situ hybridization technologist. When appropriate, they will implement the transfer to investigators' laboratories techniques through training of researchers, technicians, and monitoring of their work. The CSC will also develop new technology in response to and in anticipation of, research needs that will evolve in the U54. Examples include: 1) double-antibody labeling technique and double-antibody/in situ labeling technique, 2) confocal microscopy, 3) acquisition of reagents and techniques for human translational and murine reproduction research. External consultants have been recruited to serve the CSC to support this endeavor. The Core will be adapted to meet the changing research needs of the new U54 context. The P30-CSC unit has successfully provided quality service to a growing number of reproductive biology researcher, responsible for the completion of over 50 research projects and acquisition of numerous NIH grant proposals.