STUDY OF INTESTINAL CELL DIFFERENTIATION IN VITRO

该提案的长期目标是定义蜂窝 调节细胞增殖、分化和基因表达的过程， 肠子基本前提是细胞周期蛋白依赖性激酶 当肠干细胞变成激活的隐窝时，抑制剂被激活 细胞一种这样的细胞周期蛋白依赖性激酶抑制剂是p27，其可以作用于 独立于G1细胞周期蛋白和cdk。此外，多个冗余基因 控制增殖和分化，除了p27。因此 拟议的研究将集中在抑制p27，以评估对 为了阐明Jab 1在p27调节中的作用， 表达;将p27的分化诱导特性与 蛋白质的亚结构域;以鉴定p27相互作用蛋白，并确定 它们在体外和体内的表达;并继续鉴定新的 调节肠道分化的基因。

The long-term goal of the proposal is to define the cellular processes that regulate proliferation, differentiation, and gene expression in the intestine. The fundamental premise is that cyclin-dependent kinase inhibitors are activated when intestinal stem cells become activated crypt cells. One such cyclin-dependent kinase inhibitor is p27 which may act independently from G1 cyclins and cdks. In addition, multiple redundant genes control proliferation and differentiation, apart from p27. Therefore, the proposed studies will focus upon inhibiting p27 to assess effects upon differentiation; to elucidate the role of Jab 1 in the regulation of p27 expression; to correlate differentiation-inducing properties of p27 with a subdomain of the protein; to identify p27 interacting proteins and to determine their expression in vitro and in vivo; and to continue identification of novel genes that regulate intestinal differentiation.