CORE--CELLULAR AND MOLECULAR MORPHOLOGY

细胞和分子形态学核心（CMMC）自SDRC开始在我们的机构存在。它一直是一个被大量利用的核心，为大量的SDRC教师及其同事提供各种各样的服务。在目前的提案中，CMMC的主要目标是继续为SDRC研究人员提供最先进的服务和专业知识，使用分子探针进行各种形态和分子生物学研究，其中微观分析起着核心作用。这些服务包括提供(i)形态学研究的免疫定位和原位杂交方法，（ii）包埋、切片和染色设施和服务，（iii）旨在促进互动和协作的集中资源中心，（iv）扫描和透射电子显微镜和免疫电子显微镜服务，(v)共聚焦激光显微镜，（vi）激光捕获显微镜，（vii）由专家指导的一系列显微技术的实践培训；（viii）用于存放1,000个人体组织样本库并对其进行分类的设施；（ix）从这些组织标本中提取核酸，以最大限度地提高SDRC教师从特定皮肤病中获取DNA和RNA的机会。核心目标包括提供仪器，技术专长和实践培训，在形态学研究中使用分子探针。另一个目标是促进SDRC成员之间的技术信息交流，并鼓励资源共享。这个核心设施的主要特点是它能够灵活地适应SDRC教师不断变化的需求，以及它全面的服务范围，旨在支持包括细胞和细胞外分子在内的拟议和未来SDRC相关形态学研究的需求。负责CMMC运营的SDRC教师作为一个整合良好的团队，在核心功能的各个方面共同提供最先进的专业知识，并在装备精良的研究实验室提供训练有素的人员。

The Cellular and Molecular Morphology Core (CMMC) has existed since the beginning of the SDRC at our institutions. It has been consistently a heavily utilized Core providing a diverse array of services to a large number of SDRC faculty and their associates. In the current proposal, the principal aim of the CMMC is to continue to provide state-of-the-art services and expertise to SDRC investigators using molecular probes for a wide variety of morphological and molecular biological studies in which microscopic analysis plays a central role. These services include providing (i) immunolocalization and in situ hybridization methods for morphological studies, (ii) embedding, sectioning, and staining facilities and services, (iii) a centralized resource center that is designed to foster interaction and collaboration, (iv) scanning and transmission electron microscopy and immunoelectron microscopy services, (v) confocal laser microscopy, (vi) laser capture microscopy, (vii) expert-guided hands-on training in a range of microscopic technologies, (viii) a facility to house and catalog our 1,000 specimen human tissue sample library; and (ix) extraction of nucleic acids from those banked tissue specimens to maximize SDRC faculty access to DNA and RNA from specific skin diseases. The goals of the core including providing instrumentation, technical expertise and hands-on training in using molecular probes in morphological studies. An additional goal is to foster exchange of technical information among SDRC members and to encourage the sharing of resources. Key features of this core facility are its flexibility in adapting to the evolving needs of SDRC faculty, and its comprehensive range of services designed to support the needs of both proposed and future SDRC-related morphological studies involving cellular and extracellular molecules. The SDRC faculty responsible for CMMC operations function as a well-integrated team that collectively offers state-of-the-art expertise in all aspects of core function, and provides access to highly trained personnel in very well equipped research laboratories.