CORE--MOLECULAR BIOLOGY

凯斯西储大学分子生物学核心实验室（MBCL） 大学已经运作了10年。的指导 原则是提供最先进的科学能力， 克利夫兰的研究社区，特别强调以下几点 支持团体：CFAR，癌症中心和亲属研究中心。关于MBCL 是房子在空间装修于1990年专门为其使用，并提供 以下服务：蛋白质测序，氨基酸分析，DNA 合成，DNA测序，磷光成像，扫描激光密度测定， 生物发酵和荧光成像。MBCL的仪器具有 通过共享仪器赠款（NIH和VA）获得，私人 基金会和中心赠款（CFAR）。在过去的五年里，MBCL 化验所购置了价值约五十万元新仪器， 在某些情况下已经取代了旧的仪器（DNA合成器， 蛋白质测序仪），但在大多数情况下，提供新的方法。 在目前的申请中，要求提供资金， 生物传感器BIAcore。目前，MBCL的大部分成本是 由为每项服务确定的用户费支付，以反映 服务真实的成本（维护合同、MBCL的努力 工作人员、化学品和硬件或软件的适当升级）。的 MBCL已经运行了相当长的一段时间， 确定使用费的费率。这些费率至少要经过审查 在大多数情况下，利率要么保持不变，要么 降低这是MBCL的做法，让每项服务支付 没有任何一个服务需要通过其他服务来支持。如果 对特定服务的需求被认为太少，服务可能会被 考虑淘汰。已采用 比较HIV 1和RSV蛋白酶（Jonathan Leis，Ph.D CA 52047）的氨基酸序列和糖基化模式的分析。 gp 120的V3区（Susan Eshleman，Ph.D. HD34348）; HIV-1逆转录酶/tRNAlys复合物（StuartF. J. LeGrice AI 31147）;保护性和诊断性分枝杆菌抗原（R.沃利斯， AI24298）。

The Molecular Biology Core Laboratory (MBCL) at Case Western Reserve University has been in operation for the past 10 years. The guiding principle has been to provide state-of-the-art scientific capabilities to the research community of Cleveland with special emphasis to the following supporting groups: CFAR, Cancer Center and kin Research Center. The MBCL is house in space renovated in 1990 specially for its use and offers the following services: protein sequencing, amino acid analysis, DNA synthesis, DNA sequencing, phosphorimaging, scanning laser densitometry, biofermentation, and fluorimaging. The instrumentation for the MBCL has been obtained through shared instrumentation grants (NIH, and VA), private foundations and center grants (CFAR). In the past five years, the MBCL laboratory has purchase about $500,000 worth of new instrumentation which in some instances has been to replace old instruments (DNA synthesizer, protein sequencers), but in most instances to provide new methodologies. In the current application, funds are requested to purchase a Pharmacia Biosensor BIAcore. At the present time, most of the costs of the MBCL are covered by user fees which are established for each services to reflect the real cost of that service (maintenance contract, effort of the MBCL staff, chemicals and upgrades of hardware or software as appropriate). The MBCL has been in operation for a considerable length of time and is used to establishing rates for user fees. These rates are reviewed at least semi-annually and in most instances, the rates are either maintained or reduced. It is the practice of the MBCL to have each service pay for itself and not have any one service require support through others. If the need for a specific service is ever deemed too minimal, the service may be considered for elimination. Key AIDS-related projects which have used the facility include: Comparing HIV 1 and RSV Proteases (Jonathan Leis, Ph.D CA52047); Analysis of amino acid sequences and glycosylation patterns of the V3 region of gp120 (Susan Eshleman, Ph.D. HD34348); Crystallization of HIV-1 reverse transcriptase/tRNAlys, complexes (Stuart F.J. Le Grice AI31147); Protective and diagnostic Mycobacterial antigens (R. Wallis, AI24298).