Development and Characterization of a Synthetic Clock

描述(由申请人提供)：使用特征明确的抄写 来自大肠杆菌的因子，我们已经构建了一个人造遗传时钟 在大量细菌中产生同步振荡输出。在……里面 拟议的工作、生理学和遗传学方法将用于研究 合成钟的功能，找出重要的设计特点 时钟，并更好地使合成遗传系统免受干扰 通过其他细胞信号转导系统。建议进行生物化学研究 测量时钟功能所涉及的关键参数，并从理论上 研究将开发时钟功能的模型。建模将用于 确定影响时钟功能的参数之间的关系，并 设计更好的时钟。理论研究的预测将得到检验。 试验性的。通过比较理论研究的结果和 实验，我们将阐明系统设计的基本原则 人工基因时钟的功能。这些研究将探索 利用合成遗传系统研究系统设计的有用性 原理，发展构建合成基因的技术 系统，并促进我们对基因的时间调节的理解 表情。

DESCRIPTION (provided by the applicant): Using well-characterized transcription factors from E. coli, we have constructed a synthetic genetic clock that produces a synchronous oscillating output in large populations of bacteria. In the proposed work, physiological and genetic approaches will be used to study the function of the synthetic clock, identify the important design features of the clock, and better insulate the synthetic genetic system from interference by other cellular signal transduction systems. Biochemical studies are proposed to measure the key parameters involved in clock function, and theoretical studies will develop models of clock function. Modeling will be used to determine the relationship of parameters affecting clock function, and to design better clocks. The predictions of theoretical studies will be tested experimentally. By comparing the results of theoretical studies and experimentation, we will elucidate the system design principals underlying the function of the synthetic genetic clock. These studies will explore the usefulness of using synthetic genetic systems to study system design principles, develop the technology for the construction of synthetic genetic systems, and advance our understanding of the temporal regulation of gene expression.