WORKSHOPS--MOUSE MUTAGENESIS

描述（由申请人提供）：我们建议小型，密集的年度研讨会，其使命是协调小鼠遗传，基因组，生物和生物信息学研究和资源，以实现功能注释小鼠基因组DNA序列的10年目标。这一目标将通过以下方式实现：（1）在胚胎干细胞、小鼠或两者中的基因组中的每个基因中产生至少一个可遗传的突变;（2）鉴定影响生物医学感兴趣的关键性状的每个基因;（3）建立用于保存和分布突变细胞、小鼠和配子的基础设施;以及（4）增强对这些功能研究的信息学和数据库支持。这些讲习班将邀请来自美国、加拿大、欧洲、日本和澳大利亚的学术和商业专家参加。 讲习班的具体目标是 - 协调胚胎干细胞、配子和小鼠的生产、鉴定、存档和分配; - 通过与特定生物学领域的专家合作，开发新功能变体的第二代表型检测; - 评估大型和小型诱变项目的进展、问题和解决方案; - 在一个集中的、可公开访问的数据库中协调管理这些突变小鼠的遗传和表型信息; - 评估大型生物医学研究团体对突变小鼠的使用和效用。

DESCRIPTION (provided by applicant): We propose small, intensive annual workshops whose mission is to coordinate mouse genetic, genomic, biological and bioinformatics research and resources towards the 10-year goal of functionally annotating the DNA sequence of the mouse genome. This goal will be accomplished by (1) producing at least one heritable mutation in ES cells, mice or both in every gene in the genome; (2) identifying every gene that affects key traits of biomedical interest; (3) establishing an infrastructure for preserving and distributing mutant cells, mice and gametes; and (4) enhancing the informatics and database support for these functional studies. The workshops will involve invited academic and commercial experts from the United States, Canada, Europe, Japan and Australia. The specific aims of the workshops are - To coordinate the production, characterization, archival and distribution of ES cells, gametes and mice; - To develop 2nd generation phenotypic assays for novel functional variants by working with experts in particular biological areas; - To assess progress, problems and solutions that are being addressed in the large and small mutagenesis programs; - To coordinate the management of genetic and phenotypic information about these mutant mice in a centralized, publicly accessible database; - To evaluate the usage and utility of mutant mice by the larger biomedical research community.