Development of Debaryomyces hansenii as a robust host for production of high value fatty acid-based biochemicals.

石油化学衍生的生物化学品在全球范围内使用，然而，世界各地都在推动用可持续的生物化学品取代它们。自然界提供了许多工具来合成各种各样的生物化学物质，但在工业化收获这些化合物时出现了一些问题，如产量低、致病性和难以培养这些生物。工业生物技术社区经常面临的挑战是如何操纵细胞合成机器以生产出可以最佳使用的产品。在这个合成生物学项目中，您将成为一个跨学科团队的一员，该团队对使用非致病酵母Debarymyceshansenii生产一类生物乳化剂感兴趣。你将讲述抗性细胞如何适应化合物的产生，这些化合物可能会乳化它们的细胞膜。你将把产生乳化剂的基因引入汉森乳杆菌，然后使用包括转录分析、脂类组学和代谢途径分析在内的各种技术来解决这个问题。然后，你的发现将被用于重新设计细胞生物合成途径，以提高生物乳化剂的生产。该项目以工业生物技术原理为基础，但创造了与分子生物学家、生物信息学家、化学家和生理学家合作的机会。

Petro-chemically derived biochemicals are used globally, however,there is a worldwide drive to replace them with sustainable,biological counterparts. Nature provides many tools to synthesise ahuge variety of biochemicals but there are problems which arisewhen industrially harvesting these compounds such as low yields,pathogenicity and difficulty in culturing the organisms. Thechallenge frequently faced by the industrial biotechnologycommunity is how to manipulate cellular synthesis machinery inorder to yield products which can be used optimally.In this synthetic biology project you will be part of aninterdisciplinary team that is interested in the production of a classof bio-emulsifiers using the non-pathogenic yeast Debaryomyceshansenii as production host. You will address how the marinestress resistant cells adapt to the production of compounds thatpotentially emulsify their membranes. You will introduce the genesto produce the emulsifiers into D. hansenii and then address thequestion using a variety of techniques including transcriptomeanalysis, lipidomics and metabolic pathway analyses. Your findingswill then be used to re-engineer the cellular biosynthesis pathwayto improve bio-emulsifier production. This project is based aroundprinciples of industrial biotechnology but creates opportunities towork together with molecular biologists, bioinformaticians, chemistsand physiologists.