Nutritional Profile Optimisation of CO2 Fixing Bacteria Strains by Molecular Engineering

深科生物科技有限公司(DBB)拥有一个固定二氧化碳的细菌菌株库，这些菌株可以通过气体发酵回收工业二氧化碳废物排放，最终产生蛋白质来源。与大豆和鱼粉等现有蛋白质来源相比，这种蛋白质来源为水产养殖和牲畜提供了更可持续的替代饲料成分。在工业规模上优化DBB菌株的特性，如底物利用率、营养质量和蛋白质产量，有利于提高产品价值和加工效率。然而，为了操纵DBB菌株来执行这样的菌株改良，由于缺乏可用于工程DBB菌株的现有分子工具，必须采用回归基础的方法。因此，该项目的主要目标包括建立一个有效的分子基因系统，以促进菌株工程，并相应地利用衍生的系统，主要专注于成功地优化DBB菌株的营养图谱。在项目进展过程中，可能还会考虑扩展到优化蛋白质产量和替代产品的可能性。

Deep Branch Biotechnology Ltd (DBB) owns a library of CO2 fixing bacteria strains that can recycle industrial CO2 waste emissions, via gas fermentation, to ultimately produce a protein source. This protein source delivers a more sustainable alternative feedstock component for aquaculture and livestock compared to currently available protein sources, such as soy and fishmeal. Optimising the properties of DBB strains such as substrate utilisation, nutritional quality and protein yield, at an industrial scale confers advantageous improvements to product value and process efficiency. However, to manipulate DBB strains to execute such strain improvements, a back to basics approach must be applied due to the lack of pre-existing molecular tools available to engineer DBB strains. Hence, the main objectives of the project involve the establishment of an effective molecular gene system to facilitate strain engineering and accordingly harness the system derived to principally focus on the successful optimisation of DBB strain nutritional profiles. The potential for extension to encompass optimisation of protein yield and alternative products may be additionally considered during project progression.