Non-reentrant arrhythmias: ectopic nexus hypothesis

描述（由申请人提供）：本补充申请的主要目标是：1。通过培训课程和内部实验获得处理人类胚胎细胞（hESC）的理论和实践经验。2.开发允许从i）未定型hESC和ii）从跳动的胚胎体（EB）分离的心脏样hESC形成心肌细胞网络的方案。3.评估从hESC衍生的心肌细胞网络的脉冲传播和兴奋性特性。4.目的探讨人胚胎干细胞来源的心肌细胞网络异位心律失常的发生和发展。总的来说，我们将提出类似于我们的父R 01基金（HL 076722，“非折返性心律失常的起源：异位联系假说”，04/01/04-03/31/08）中提出的问题，但与来自hESC的网络有关。该项目将使我们能够了解大量关于潜在心脏移植物的电特性和致心律失常性。拟定实验将使用NIH人类胚胎干细胞注册表中列出的人类ES细胞系进行，NIH代码：WA 01和WA 09（提供商代码：H1和H9）。

DESCRIPTION (provided by applicant): The main goals of this supplemental application are: 1. To gain theoretical and practical experience dealing with human embryonic cells (hESC) both through training courses and by performing in-house experiments. 2. To develop the protocols that will allow formation of the networks of cardiomyocytes from i) uncommitted hESC and ii) cardiac-like hESC isolated from beating embryonic bodies (EB). 3. To assess the impulse propagation and excitability properties of the cardiomyocyte networks derived from the hESC. 4. To address the occurrence and development of ectopic arrhythmias in the cardiomyocyte networks derived from the hESC. Overall, we will be asking questions similar to the ones posed in our parent R01 grant (HL 076722, "Origins of non-reentrant arrhythmias: ectopic nexus hypothesis", 04/01/04-03/31/08), but in relation to the networks derived from the hESC. The project will allow us to learn a great deal about the electrical properties and arrhythmogenicity of prospective cardiac grafts. The proposed experiments will be conducted using human ES cell lines listed in the NIH Human Embryonic Stem Cell Registry with NIH Code: WA01 and WA09 (Provider's Code: H1 and H9).