Biomimetic Nano Scaffolds

描述（由申请人提供）：修复慢性炎症性疾病牙周炎破坏的牙齿支撑结构是口腔重建治疗的主要目标。我们建议开发一种新的支架系统，可以将再生剂输送到牙周缺损。该系统由纳米纤维聚合物支架组成，支架用骨矿物模拟磷灰石修饰，含有微球，用于递送生物活性分子，如骨形态发生蛋白-7 （BMP-7）。预计这种支架/因子输送系统将通过提供一个环境来增强骨母细胞、骨水泥母细胞和牙周韧带（PDL）成纤维细胞的粘附、迁移和分化，从而促进缺陷部位的牙周再生。此外，这种支架输送系统将允许营养物质、代谢物和三维（3D）组织新生所需的信号分子的渗透。这个探索性的研究项目将集中于支架/因子传递系统的开发，并测试这种新方法用于治疗牙周缺陷的可行性。以下具体目标旨在产生初步数据，以确定该方法用于牙周组织工程的强度。具体目标设计具有相互连接的球形大孔和骨矿物样磷灰石修饰的纳米纤维支架。具体目标2。将含有BMP-7的微球整合到纳米纤维支架中，体外评价BMP-7的生物活性和释放动力学。完成这些具体目标将产生关键的初步数据，并为设计最佳牙周再生疗法使用适当的支架/因子输送系统提供重要信息。这些知识将使我们能够开发出可预测的体内应用仿生支架，并将成为我们计划的R01研究的基础。

DESCRIPTION (provided by applicant): Repair of tooth-supporting structures destroyed by the chronic inflammatory disease periodontitis is a major goal of oral reconstructive therapy. We propose to develop a novel scaffolding system that can deliver regenerative agents to periodontal defects. This system consists of a nano-fibrous polymer scaffold modified with bone mineral-mimicking apatite containing microspheres for delivery of bioactive molecules such as bone morphogenetic protein-7 (BMP-7). It is anticipated that this scaffolding/factor delivery system will promote periodontal regeneration at the defect site by providing an environment for enhancing adhesion, migration, and differentiation of putative cells such as osteoblasts, cementoblasts, and periodontal ligament (PDL) fibroblasts. Moreover, this scaffolding delivery system will allow for permeation of nutrients, metabolites, and signaling molecules required for three dimensional (3D) tissue neogenesis. This exploratory research project will focus on development of the scaffolding/factor delivery system and on testing the feasibility of this novel method for use in treatment of periodontal defects. The following specific aims are designed to generate preliminary data to determine the strength of this approach for periodontal tissue engineering. Specific Aim 1. Design nano-fibrous scaffolds with interconnected spherical macropores and modified with bone mineral-like apatite. Specific Aim 2. Integrate microspheres containing BMP-7 into nano-fibrous scaffolds and evaluate the bioactivity and release kinetics of BMP-7 in vitro. Accomplishing these specific aims will generate critical preliminary data and provide vital information as to the appropriate scaffolding/factor delivery system for use in designing optimal periodontal regenerative therapies. This knowledge will enable us to develop a predictable biomimetic scaffold for in vivo application and will be the basis for our planned R01 investigation.