Development of a whole cell biocatalyst designed to perform hydrogen-dependent carbon dioxide reduction under pressure.

该项目将专注于开发和表征一种细菌碳捕获系统，该系统利用复合甲酸氢解酶（FHL）从气态二氧化碳和H2中生成甲酸，同时细胞在加压反应器中生长。开发的系统还需要能够在一氧化碳存在的情况下工作。该项目的主要目的是：表征大肠杆菌中萎缩Pectobacterium atrospeticum细菌甲酸氢解酶（FHL-2）酶复合物的活性。为了验证FHL-2在大肠杆菌中完全起作用的假设，并且可以作为氢依赖的二氧化碳还原酶（HDCR）以气态氢和二氧化碳的形式生成甲酸盐。设计、组装和表征大肠杆菌中合成的一氧化碳脱氢酶（CODH）酶复合物。本节旨在验证一个假设，即来自Pantoea细菌的CODH可以在大肠杆菌中成功表达，并且可以从一氧化碳中产生二氧化碳。组装和表征一种新型的氢依赖性二氧化碳还原细胞生物催化剂。本节旨在验证一个假设，即在表达FHL-2的细菌系统中添加活性CODH将产生一种生物催化剂，能够在一氧化碳存在的情况下还原氢依赖的二氧化碳。

The project will focus on developing and characterising a bacterial carbon capture system that utilises the enzyme complex formate hydrogenlyase (FHL) to generate formate from gaseous CO2 and H2 whilst cells grow in a pressurised reactor. The developed system will also need be capable of functioning in the presence of carbon monoxide. The key aims of this project are:To characterise the activity of the formate hydrogenlyase (FHL-2) enzyme complex from the bacterium Pectobacterium atrospeticum in Escherichia coli.To test the hypothesis that FHL-2 is fully functional within E. coli and can operate as a hydrogen-dependent CO2 reductase (HDCR) to generate formate form gaseous hydrogen and carbon dioxide.To design, assemble and characterise a synthetic Carbon Monoxide Dehydrogenase (CODH) enzyme complex in E. coli.This section aims to test the hypothesis that the CODH from a Pantoea species of bacteria can be successfully expressed in E. coli and can generate carbon dioxide from carbon monoxide.To assemble and characterise a novel hydrogen-dependent carbon dioxide reducing cell-based biocatalyst. This section aims to test the hypothesis that the addition of an active CODH into a bacterial system expressing FHL-2 will produce a biocatalyst capable of hydrogen-dependent carbon dioxide reduction in the presence of carbon monoxide.