Biomimetic Nano Scaffolds

描述（由申请人提供）：修复被慢性炎症性疾病牙周炎破坏的牙齿支撑结构是口腔重建治疗的主要目标。我们建议开发一种新的支架系统，可以提供再生剂牙周缺损。该系统由纳米纤维聚合物支架组成，该支架用含有微球的骨矿物质模拟磷灰石修饰，用于递送生物活性分子，如骨形态发生蛋白-7（BMP-7）。预计这种支架/因子递送系统将通过提供用于增强假定细胞（例如成骨细胞、成牙骨质细胞和牙周韧带（PDL）成纤维细胞）的粘附、迁移和分化的环境来促进缺损部位的牙周再生。此外，该支架递送系统将允许三维（3D）组织新生所需的营养物、代谢物和信号分子的渗透。这个探索性的研究项目将集中在支架/因子输送系统的开发和测试这种新方法用于治疗牙周缺损的可行性。以下具体目标旨在生成初步数据，以确定牙周组织工程这种方法的强度。具体目标1。设计具有相互连接的球形大孔的纳米纤维支架，并用骨矿物样磷灰石修饰。 具体目标2。将含BMP-7的微球整合到纳米纤维支架中，并在体外评价BMP-7的生物活性和释放动力学。 实现这些具体目标将产生关键的初步数据，并提供重要的信息，以适当的支架/因子输送系统，用于设计最佳的牙周再生治疗。这些知识将使我们能够开发一种可预测的仿生支架用于体内应用，并将成为我们计划的R 01研究的基础。

DESCRIPTION (provided by applicant): Repair of tooth-supporting structures destroyed by the chronic inflammatory disease periodontitis is a major goal of oral reconstructive therapy. We propose to develop a novel scaffolding system that can deliver regenerative agents to periodontal defects. This system consists of a nano-fibrous polymer scaffold modified with bone mineral-mimicking apatite containing microspheres for delivery of bioactive molecules such as bone morphogenetic protein-7 (BMP-7). It is anticipated that this scaffolding/factor delivery system will promote periodontal regeneration at the defect site by providing an environment for enhancing adhesion, migration, and differentiation of putative cells such as osteoblasts, cementoblasts, and periodontal ligament (PDL) fibroblasts. Moreover, this scaffolding delivery system will allow for permeation of nutrients, metabolites, and signaling molecules required for three dimensional (3D) tissue neogenesis. This exploratory research project will focus on development of the scaffolding/factor delivery system and on testing the feasibility of this novel method for use in treatment of periodontal defects. The following specific aims are designed to generate preliminary data to determine the strength of this approach for periodontal tissue engineering. Specific Aim 1. Design nano-fibrous scaffolds with interconnected spherical macropores and modified with bone mineral-like apatite. Specific Aim 2. Integrate microspheres containing BMP-7 into nano-fibrous scaffolds and evaluate the bioactivity and release kinetics of BMP-7 in vitro. Accomplishing these specific aims will generate critical preliminary data and provide vital information as to the appropriate scaffolding/factor delivery system for use in designing optimal periodontal regenerative therapies. This knowledge will enable us to develop a predictable biomimetic scaffold for in vivo application and will be the basis for our planned R01 investigation.