Development of Infection-Resistant Suture Materials

描述(由申请人提供)： 感染仍然是使用生物材料的主要并发症之一。在美国240万医院感染中，手术部位感染约占14-16%，这些感染导致患者发病率和死亡率增加。各种生物材料固有的体积特性，包括那些包含缝合线的材料，为最初的细菌与随后的生物膜生产和生长提供了一个环境。一旦病原体(S)附着在生物材料表面，抗菌剂的治疗就会无效，因为抗菌剂对细菌生物膜的渗透有限。因此，新型抗感染缝合线生物材料的开发将在材料表面和周围组织提供一个杀菌环境。这一阶段项目的目标是通过使用纺织品染色技术来应用苯环结构抗生素环丙沙星、多西环素和利奈唑胺，开发抗感染的尼龙、丝绸和涤纶(涤纶)体外缝合材料，并具有最佳的抗菌性能。我们的假设是基于苯环的抗生素可以用来“染色”生物医学上有用的缝合材料。此外，这种吸收可能会被优化，由此产生的处理材料将在较长时间内具有缓慢、持续的抗生素释放，从而提供具有更好的抗感染能力的临床有用的缝合材料。我们初步研究的数据支持这些假设。本研究的具体目的是：1)优化尼龙、丝绸和涤纶缝合线材料的抗菌染色条件；2)表征抗生素染色缝合线材料的物理性能；3)用分光光度法测定静态/水洗条件下抗生素的释放；4)检测静态和洗涤抗生素染色缝合线材料的抗菌活性。根据目前的感染率和治疗这些患者的费用(每一次2,300美元)，手术伤口感染导致医疗保健系统每年的成本超过7.5亿美元。因此，应用我们的技术来预防伤口感染是一个巨大的市场。将在第二阶段完成的长期目标将是在体内评估这项技术，以便确定伤口损伤模型中的感染抵抗力。

DESCRIPTION (provided by applicant): Infection remains as one of the major complications associated with utilizing biomaterials. Surgical site infections account for approximately 14-16% of the 2.4-million nosocomial infections in the United States, with these infections resulting in increased patient morbidity and mortality. The inherent bulk properties of various biomaterials, including those that comprise sutures, provide a milieu for initial bacterial adhesion with subsequent biofilm production and growth. Once the pathogen(s) adheres to the biomaterial surface, treatment with antimicrobial agents is ineffective due to limited penetration of the agent through the bacterial biofilm. Thus, development of a novel infection-resistant suture biomaterial would provide a bacteriocidal environment at the material surface as well as in the surrounding tissue. The goal of this phase I project is to develop infection-resistant nylon, silk and polyester (Dacron) suture materials in vitro with optimum antimicrobial properties by employing textile-dyeing techniques to apply the benzene ring-structured antibiotics Ciprofloxacin (Cipro), Doxycycline and Linezolid. Our hypotheses are that benzene ring-based antibiotics can be used to "dye" biomedically-useful suture materials. Additionally, this uptake may be optimized, and that the resulting treated material will possess a slow, sustained release of antibiotic over a prolonged period of time, thereby providing clinically useful suture materials with improved infection-resistance. Data from our preliminary studies supports these hypotheses. The specific aims of this study are to: 1) optimize antibiotic dyeing conditions to nylon, silk and Dacron suture materials, 2) characterize the physical properties of the antibiotic-dyed suture materials, 3) determine antibiotic release under static/washing conditions via spectrophotometry and 4) examine antimicrobial activity of static and washed antibiotic-dyed suture materials. Based on the current infection rates in conjunction with the costs to treat these patients ($2,300/episode), surgical wound infection results in an annual cost to the healthcare system of greater than $750 million. Thus, a significant market exists for application of our technology in order to prevent wound infection. The long-term goal, which will be completed in phase II, will be to assess this technology in vivo in order to determine infection-resistance in a wound injury model.