CALCIUM AND ENDOCYTOTIC MEMBRANE RETRIEVAL

描述：（申请人摘要） 内吞膜修复补偿多余的表面膜 但胞吐-胞吞耦合的机制是 不知道。我们已经在海胆卵中发现， 钙通过对蛇曲霉毒素敏感的通道内流。除此之外， 在突触胞吐信号中的作用，P型钙通道是 这是卵细胞内吞膜修复所必需的。我们假设 胞吐调节P型钙通道门控以协调胞吐， 胞吞膜修复我们将使用显微镜，电生理学， 以及细胞和分子生物学技术，以确定如何胞吐 活性调节海胆卵中通过P型通道的钙内流， 了解钙触发的胞吐和胞吞作用的模型系统。 具体地说，我们将确定胞吐活动如何影响膜 去极化和P型钙通道的细胞分布。

DESCRIPTION: (Applicant's Abstract) Endocytotic membrane retrieval compensates for excess surface membrane following exocytosis but the mechanism of exocytosis-endocytosis coupling is not known. We have shown in sea urchin eggs that membrane retrieval requires calcium influx through agatoxin sensitive channels. Thus, in addition to their role in signaling for exocytosis at synapses, P-type calcium channels are required for endocytotic membrane retrieval in eggs. We hypothesize that exocytosis regulates P-type calcium channel gating to coordinate exocytosis and endocytotic membrane retrieval. We will use microscopy, electrophysiology, as well as cell and molecular biological techniques to determine how exocytotic activity regulates calcium influx through P-type channels in sea urchin eggs, a model system for understanding calcium-triggered exocytosis and endocytosis. Specifically we will determine how exocytotic activity influences membrane depolarization and the cellular distribution of P-type calcium channels.