Multi-Laser Flow Cytometer

描述（由申请人提供）：该多激光流式细胞仪将升级和扩展位于Beth Israel Deaconess医疗中心的现有的、充分利用的研究流式细胞仪核心设备的能力。这个研究流式细胞仪核心设施由一个忠诚的，训练有素的全职经理负责培训和监督研究人员，监督单激光流式细胞仪和操作双激光荧光激活细胞分选器。这个核心设施自1988年以来一直在运行，现在被位于贝斯以色列女执事医疗中心和哈佛医学院地区毗邻机构的众多不同研究小组完全利用。根据这些研究小组目前的需求，所要求的额外仪器将使流式细胞仪设施中的共享仪器能够提供新的，目前不可用的分析类型，并将减轻目前常规使用单一的，广泛使用的流式细胞仪/细胞分选仪的延误。所要求的仪器包括一台四激光研究流式细胞仪，这是一种提高研究流式细胞仪可用性的经济有效的手段。双激光细胞分选机升级为多参数细胞分选，充分利用了时间。所要求的仪器将提供多参数分析能力，包括多色染色和细胞周期和钙通量分析，这些分析越来越需要但却无法获得。作为一个共享的、专用的多用户研究资源，贝斯以色列女执事医疗中心的研究流式细胞仪设备已经并将继续提供其他方面无法获得的研究支持能力。使用该设施的研究人员包括贝斯以色列女执事医疗中心各部门的许多研究实验室的研究人员，包括内科、病理学、外科和神经内科，以及其他附近机构的研究人员，包括儿童医院医疗中心和布里格姆妇女医院。目前和计划中的基金支持的研究包括免疫学、炎症、分子生物学、细胞生物学、过敏、血液学、肿瘤学、风湿病学、肺部疾病、胃肠道疾病、心血管疾病和病毒感染相关的研究。

DESCRIPTION (provided by applicant): The multi-laser flow cytometer will upgrade and expand the capabilities of an existing, fully utilized Research Flow Cytometry Core Facility located at the Beth Israel Deaconess Medical Center. This Research Flow Cytometry Core Facility is staffed by a committed, trained full-time Manager who trains and supervises researchers, oversees a single laser flow cytometer and operates a dual laser fluorescence-activated cell sorter. This Core Facility, which has been operational since 1988, is now completely utilized by a multitude of diverse research groups located both within the Beth Israel Deaconess Medical Center and at adjoining institutions in the Harvard Medical School area. Based on the current needs of these many research groups, the requested additional instrumentation would enable the shared instrumentation in the Flow Cytometer Facility to provide new, presently unavailable types of analyses and would alleviate the delays now routine with the single, extensively utilized flow cytometer/cell-sorter. The requested instrumentation includes a four-laser research flow cytometer, which represents a cost effective means of enhancing the availability of research flow cytometry. The dual laser cell sorter has been upgraded for multi-parameter cell sorting, which fully utilizes its time. The requested instrument would provide multi-parameter analytic capabilities, including multi-color staining and cell cycle and calcium flux assays that are increasingly needed and yet unavailable. As a shared, dedicated multi-user research resource, the Research Flow Cytometry Facility at the Beth Israel Deaconess Medical Center has provided, and will continue to provide, research support capabilities not otherwise available. Investigators utilizing this Facility include those in many research laboratories in Departments at the Beth Israel Deaconess Medical Center, including the Departments of Medicine, Pathology, Surgery, and Neurology, and investigators at other nearby institutions, including the Children's Hospital Medical Center and the Brigham and Women's Hospital. The research studies supported by the current and planned Facility include those related to immunology, inflammation, molecular biology, cell biology, allergy, hematology, oncology, rheumatology, pulmonary diseases, gastrointestinal diseases, cardiovascular diseases, and viral infections.