Biacore 3000 SPR Instrument

描述（由申请人提供）：该共享仪器资助要求使用Biacore 3000仪器对受体配体结合相互作用进行实时表面等离子体共振（SPR）分析。核心用户组由来自医学系（Lehrer，Ganz，科尔），病理学系（Baum，Ganz），微生物学，免疫学和分子遗传学系（Lee）和分子生物学研究所（Baum，Lehrer）的五名全职教师组成。双分子结合相互作用是所有这些研究人员研究工作的核心，所有人都将受益于所需仪器的特殊灵敏度和高通量能力。由于三位用户（Baum、Lee和Lehrer）研究肽和蛋白质与碳水化合物和糖的相互作用，因此只有Biacore 3000仪器结合了a）检测寡糖结合所需的灵敏度和B）高通量能力，以及c）以最小稀释度回收分析物样品的能力。调查人员致力于共享该仪器，并将积极主动地向其他用户提供。这将包括为仪器的临时用户或新用户提供两名高技能操作员的服务。该提案的好处和健康相关性在于，它将有助于实现表1中列出的10项NIH赠款或提案的研究目标。这些包括开发新的药物和方法来预防和治疗HIV-1和其他病毒病原体引起的感染，以及与细胞靶向和存活有关的基本问题。

DESCRIPTION (provided by applicant): This Shared Instrumentation Grant requests a Biacore3000 instrument to perform real-time surface plasmon resonance (SPR) analyses of receptor ligand binding interactions. The core user group consists of five full-time faculty members from the Departments of Medicine (Lehrer, Ganz, Cole), Pathology (Baum, Ganz), Microbiology, Immunology and Molecular Genetics (Lee), and Molecular Biology Institute (Baum, Lehrer). Biamolecular binding interactions are central to the research efforts of all of these investigators, and all will benefit from the exceptional sensitivity and high throughput capacity of the requested instrument. Because three of the users (Baum, Lee and Lehrer) study the interactions of peptides and proteins with carbohydrates and sugars, only the Biacore3000 instrument combines a) the sensitivity necessary to detect oligosaccharide binding, with b) high throughput capacity, and c) the ability to recover analyte samples with minimal dilution. The investigators are committed to sharing the instrument and will be proactive in making it available for other users. This will include providing access to the services of two highly skilled operators for occasional or new users of the instrument. The benefits and health relatedness of the proposal are that it will facilitate accomplishing the research goals of the ten NIH grants or proposals listed in Table 1 within. These include the development of new agents and approaches to prevent and treat infections caused by HIV-1 and other viral pathogens, and fundamental issues related to cell targeting and survival.