BD LSR II

描述(由申请人提供)：免疫系统在病原体清除和肿瘤监测中起着关键作用。免疫系统中的关键细胞角色包括T和B淋巴细胞以及树突状细胞，它们以多种分化和激活状态存在。它们的免疫功能在很大程度上取决于它们的状态。有大量的知识和试剂库可用于评估淋巴细胞和树突状细胞的不同发育和激活状态，特别是在小鼠身上。然而，随着知识库的发展，我们发现有必要使用更复杂的工具来评估复杂的表型。当今可用的一个强大工具是多参数流式细胞术。在本申请中描述的研究中，流式细胞术不仅用于确定细胞亚群，而且还用于研究受体-配体结合动力学、生化信号通路、细胞因子分泌、细胞周期状态和细胞在体内的运输。这个应用程序申请资金购买最先进的多参数流式细胞仪--LSR II流式细胞仪。所需的附件(4个激光)使该仪器能够收集每个电池的10个参数或“颜色”信息。这一仪器将使我们能够更好地了解淋巴细胞和树突状细胞亚群的复杂功能作用。

DESCRIPTION (provided by applicant): The immune system plays a critical role in pathogen clearance and tumor surveillance. Key cellular players in the immune system include T and B lymphocytes and dendritic cells, which exist in a multitude of differentiative and activation states. Their function in immunity critically depends on their state. There is a large knowledge and reagent base available to evaluate the different developmental and activation states of lymphocytes and dendritic cells, particularly in mice. However, as the knowledge base evolves, we find it necessary to use more sophisticated tools to evaluate complex phenotypes. A powerful tool available today is multi-parameter flow cytometry. In the studies described in this application, flow cytometry is being used not only to define cellular subpopulations, but also to study receptor-ligand binding kinetics, biochemical signaling pathways, cytokine secretion, cell cycle status, and cellular trafficking within the body. This application requests funds to purchase a state-of-the-art instrument for multi-parameter flow cytometry--the LSR II flow cytometer. The accessories requested (4 lasers) give this instrument the ability to collect 10 parameters or "colors" of information on each cell. This instrument will allow us to better understand the complex functional roles of subsets of lymphocytes and dendritic cells.