Typhoon 9410 Gel/Blot Imager

描述（由申请人提供）：申请资金用于购买一台新的台风9410凝胶/印迹成像仪，用于EVMS，该成像仪将被安置在微生物学和分子细胞生物学部门的生物医学蛋白质组学中心。核心设施将服务于整个EVMS校园。主要用户是7名NIH资助的研究者和3名NIH资助的正在评估或正在评估的研究者；他们共同代表EVMS的四个部门。在他们之间，主要用户有9个NIH拨款。首席研究员Semmes博士于2000年从弗吉尼亚大学（University of Virginia）转至EVMS。他目前是微生物学和分子细胞生物学系的副教授，也是EVMS生物医学蛋白质组学中心的研究主任。Semmes博士在使用各种定量分子方法来确定蛋白质、DNA、RNA的大分子相互作用方面拥有超过12年的经验。台风9410将对他在分子病毒学和癌症蛋白质组学方面的研究工作作出重大贡献。福加克斯女士和汉森博士将负责管理台风号的日常操作和培训要求。她和Hanson博士在蛋白质和核酸定量方面拥有超过五年的研究技术经验。要求使用这一工具的主要原因是为了便利主要用户群体的研究和今后几年将雇用的新调查人员的研究。EVMS现有的磷光成像仪不足以支持本提案中概述的实验，也不足以支持NIH未来的研究努力。为了维持和扩大NIH对主要用户群体和其他人的资助，有必要用这种新的最先进的磷光成像仪取代目前过时的磷光成像仪，这将增加NIH资助的研究人员的分子检测方法。此外，一些调查人员将能够利用台风的特征开展新的调查途径。

DESCRIPTION (provided by applicant): Funds are requested to purchase a new Typhoon 9410 Gel/Blot Imager for EVMS to be housed in the Center for Biomedical Proteomics in the Department of Microbiology and Molecular Cell Biology. The core facility will serve the entire EVMS campus. Major users are 7 NIH-funded investigators and 3 investigators with NIH funding either pending or under evaluation; together they represent 4 departments at EVMS. Between them, the major users have 9 NIH grants. The Principal Investigator, Dr. Semmes, moved to EVMS from the University of Virginia in 2000. He is currently an Associate Professor in the Department of Microbiology and Molecular Cell Biology and the Research Director of the EVMS Center for Biomedical Proteomics. Dr. Semmes has over twelve years of experience in using a variety of quantitative molecular approaches to determining macromolecular interactions of protein, DNA, RNA. The Typhoon 9410 will contribute significantly to his research efforts in molecular virology and cancer proteomics. Ms. Forgacs and Dr. Hanson, will be the individuals responsible for managing the day-to-day operation and training requirements of the Typhoon. Ms. Forgacs and Dr. Hanson have over five years of research technical experience in protein and nucleic acid quantitation. The major reason for requesting this instrument is to facilitate the research of the major user group and that of new investigators who will be hired over the next several years. The existing phosphorimager at EVMS is inadequate to support the experiments outlined in this proposal and to support future NIH research endeavors. In order to maintain and expand the NIHfunding of the major user group and others, it is necessary to replace the current antiquated phosphorimager with this new state-of-the-art phosphorimager, which will augment the molecular detection methods for the NIH funded investigators. Additionally, several investigators will be able to undertake new avenues of investigation using the Typhoon's features.