BIACORE 3000 Biosensor

描述（由申请人提供）：此共享仪器拨款申请寻求资金购买Biacore 3000生物传感器仪器。Biacore 3000使用表面等离子体共振来真实的实时监测生物分子的相互作用。这种全自动、灵敏、最先进的仪器的主要用户（75%）将是约翰霍普金斯大学彭博公共卫生学院生物化学和分子生物学系的六名研究人员。这些用户的具体项目涵盖了广泛的生物医学重要领域：1）蛋白质水解和DNA修复中的多聚泛素链信号（Pickart博士）; 2）DnaJ和DnaK分子伴侣参与底物选择和核蛋白分解反应（Dr. McMacken）; 3）SUMO修饰的调控和功能（Matunis博士），4）修复蛋白与烷基链间交联DNA的相互作用（Dr.米勒）; 5）通过Ku依赖性DNA末端连接的DNA修复（Dr. Hanakahi）;和6）通过U2辅助因子的RNA剪接位点选择（Dr. Kielkopf）。Biacore 3000上的一个重要部分（25%）的时间将留给我们部门和约翰霍普金斯大学其他部门的其他研究人员。该仪器的可用性将有力地促进约翰霍普金斯大学的多个NIH资助的研究项目。认识到这一事实，彭博公共卫生学院对Biacore 3000的长期运营和维护做出了实质性承诺

DESCRIPTION (provided by applicant): This shared instrumentation grant application seeks funds to purchase a Biacore 3000 biosensor intsrument. The Biacore 3000 uses surface plasmon resonance to monitor biomolecular interactions in real time. The major users (75%) of this fully automated, sensitive, state-of-the-art instrument will be six investigators in the Department of Biochemistry and Molecular Biology in the Johns Hopkins University Bloomberg School of Public Health. The specific projects of these users cover a broad range of biomedically important areas: 1) signaling by polyubiquitin chains in proteolysis and DNA repair (Dr. Pickart); 2) involvement of the DnaJ and DnaK molecular chaperones in substrate selection and nucleoprotein disassembly reactions (Dr. McMacken); 3) regulation and function of SUMO modification (Dr. Matunis), 4) interactions of repair proteins with alkyl interstrand cross-linked DNA (Dr. Miller); 5) DNA repair by Ku-dependent DNA end-joining (Dr. Hanakahi); and 6) RNA splice site choice by U2 auxiliary factor (Dr. Kielkopf). A significant fraction (25%) of the time on the Biacore 3000 will be set aside for other researchers in our Department and other departments at Johns Hopkins University. The availability of this instrument will strongly promote multiple NIH-funded research programs at the Johns Hopkins University. In recognition of this fact, the Bloomberg School of Public Health has made a substantial commitment to the long-term operation and maintenance of the Biacore 3000