JEOL 1230 TEM and BOC Edwards Vacuum Evaporator

描述（由申请人提供）：本提案要求为JEOL 1230透射电子显微镜（TEM）和BOC Edwards Auto 306真空蒸发器提供资金。这些仪器将位于爱荷华州大学中央显微镜研究设施。JEOL 1230之所以被要求，是因为该设施目前只有一台TEM，这是1987年用大学资金购买的日立H-7000。在过去的12个月里，78个教师实验室使用这种仪器来支持他们的研究。这些研究人员中有60人获得了NIH资助的研究赠款。每学期有8名学生参加电子显微镜技术课程并使用该仪器。研究人员必须提前2至3周报名参加日立TEM。一些运营商在晚上和周末使用TEM。由于维护的意外停机时间，无法保证仪器在预定时间内正常工作。这是由于大量使用，日立的年龄，这是一个设计在1981年推出，以及服务人员的专业知识水平低的组合。研究人员还需要低剂量成像、自动化载物台控制、断层扫描、数字成像和Cryo-TEM来研究他们的样品。日立TEM缺乏所有这些现代功能。BOC Edwards真空蒸发器将取代该设施中已有30年历史的Varian装置。15年来一直无法获得维修部件;它受到真空度差和碳和金属蒸发不一致的影响。用于薄片的碳涂层滤线栅和用于负染色、金属涂层和Cryo-TEM的碳薄膜必须非常薄且对比度低。JEOL TEM和BOC Edwards蒸发器将分别位于Eckstein医学研究大楼的84室和92室，作为爱荷华州大学中央显微镜研究设施的一部分。设施内有经验的人员将提供行政和维护以及高级别技术援助和培训。机构的支持是强大的，提供了61 234.00美元用于购买仪器，55 133.00美元用于研究助理的全额工资支持，10 000.00美元用于持续资助JEOL TEM的年度服务合同。威尔士将研究气道上皮细胞生物学，气道感染和基因转移。Apicella计划研究细菌的致病机理。戴利将检查大脑中突触的发育和损伤。McCray将参与使用慢病毒载体的基因转移。Tobacman将研究短肌动蛋白丝的结构。Zabner将检查腺相关病毒的形态。

DESCRIPTION (provided by applicant): This proposal requests funds for a JEOL 1230 Transmission Electron Microscope (TEM) and a BOC Edwards Auto306 Vacuum Evaporator. These instruments will be located in the University of Iowa Central Microscopy Research Facility. The JEOL 1230 is being requested because the facility currently has just one TEM, a Hitachi H-7000 purchased with university funds in 1987. During the past 12 months, 78 faculty labs have used this instrument to support their research. 60 of these investigators have NIH funded research grants. 8 students each semester are enrolled in an Electron Microscopy Techniques course and use this instrument. Investigators have to sign up for the Hitachi TEM 2 to 3 weeks in advance. A number of operators are using the TEM during evenings and weekends. There is no guarantee that the instrument will be functional at a scheduled period because of unexpected downtime for maintenance. This results from a combination of very heavy use, the age of the Hitachi, which is a design introduced in 1981, and the low level of expertise of service personnel. Investigators also need low dose imaging, automated stage controls, tomography, digital imaging and Cryo-TEM to study their samples. The Hitachi TEM lacks all of these modern capabilities. The BOC Edwards Vacuum Evaporator will replace a 30 year old Varian unit in the facility. Repair parts have been unavailable for over 15 years; it suffers from poor vacuum and inconsistent evaporation of carbon and metals. It is critical that carbon coated grids for thin-sections and carbon thin films for negative staining, metal coating and Cryo-TEM be very-thin and low contrast. The JEOL TEM and the BOC Edwards evaporator will be conveniently located in room 84 and 92 respectively in the Eckstein Medical research Building as part of the University of Iowa Central Microscopy Research Facility. Administration and maintenance, as well as high-level technical assistance and training, will be provided by experienced personnel within the facility. Institutional support is strong with $61,234.00 being provided towards purchase of the instruments, $55,133.00 in full salary support for a research assistant and $10,000.00 to partially fund the annual service contract for the JEOL TEM on a continuing basis. Welsh will study airway epithelial cell biology, airway infections and gene transfer. Apicella plans to investigate the pathogenesis of bacteria. Dailey will examine synapse development and injury in brain. McCray will be involved with gene transfer using lentiviral vectors. Tobacman will study the structure of short actin filaments. Zabner will examine the morphology of Adeno-associated virus.