Acquisition of Cryogenically-Cooled NMR Probe at 800 MHz

描述（由申请人提供）：杜克大学将为目前在其核磁共振中心运行的瓦里安Inova 800 MHz核磁共振（NMR）系统购买低温冷却，超高灵敏度三重共振，1H{13C115N}， 5mm探头和相关的闭式循环低温系统。低温冷却核磁共振探针和相关的前置放大器硬件已被证明可以显著改善可实现的信噪比，在涉及生物学上重要的大分子样品在毫摩尔和微摩尔浓度和小分子代谢物在纳摩尔浓度的研究中。核磁共振仪器、核磁共振实验和分子生物学的发展为研究人员提供了研究更具挑战性和更广泛的生物医学相关系统的能力，这些系统涉及大型单体和复合物、有限溶解度的大分子、模拟体内遇到的生物分子条件下的生物分子、极低浓度的代谢物和大范围时间尺度内系统的动力学。目前，位于杜克大学核磁共振中心的800 MHz系统被杜克社区和整个东南地区的大量科学家使用，以解决广泛的生物学重大项目。杜克大学生物化学和核磁共振中心的七名研究人员，以及来自北卡罗来纳大学、北卡罗莱纳州立大学、弗吉尼亚大学、NIEHS和圣裘德儿童医院的九名科学家，是这种低温冷却探针的最初主要用户。所有这些研究人员目前都参与了技术要求高的研究，旨在阐明重要的生物医学和生物大分子的结构、功能和动力学。随着研究范围扩大到技术要求更高的问题，许多研究小组都表达了对比目前传统探针更高灵敏度的需求。杜克核磁共振中心的800 MHz光谱仪增加了一个超高灵敏度低温冷却探头，这代表了该地区独特资源的重大进步：这一增加将增强已经充满活力的研究社区。

DESCRIPTION (provided by applicant): Duke University will purchase a cryogenically-cooled, ultra-high sensitivity triple resonance, 1H{13C115N}, 5 mm probe and associated closed-cycle cryogenic system for the Varian Inova 800 MHz nuclear magnetic resonance (NMR) system currently operating in its NMR Center. Cryogenically cooling an NMR probe and the associated pre-amplifier hardware has been demonstrated to provide a significant improvement in achievable signal-to-noise ratios in studies involving both samples of biologically important macromolecules at millimolar and micromolar concentrations and of small molecule metabolites at nanomolar concentrations. Developments in NMR instrumentation, NMR experimentation and molecular biology have provided researchers with the ability to investigate more challenging and, by extension, more biomedically relevant systems involving large monomers and complexes, macromolecules with limited solubility, biomolecules under conditions that mimic those encountered in-vivo, metabolites at very low concentrations and the dynamics of systems over a wide range of timescales. Currently, the 800 MHz system, located in the Duke University NMR Center, is utilized by a large contingent of scientists, both within the Duke community and across the Southeast region, to address a broad range of biologically significant projects. Seven investigators in Biochemistry and the NMR Center at Duke, along with nine scientists from the University of North Carolina, North Carolina State University, the University of Virginia, the NIEHS and St. Jude Children's Hospital constitute the initial majors users of the cryogenically-cooled probe. All of these investigators are currently involved in technically demanding research aimed at elucidating the structure, function and dynamics of important biomedical and biological macromolecules. The need for even higher sensitivity than currently offered by conventional probes has been expressed by many of these groups as they extend their research to more technically demanding problems. The addition of an ultra-high sensitivity cryogenically cooled probe to the 800 MHz spectrometer in the Duke NMR Center represents a major advance to a unique resource in the region: an addition that will enhance an already vibrant research community.